Rabbit anti-Human Vacuolar Protein Sorting 26 Polyclonal Antibody | anti-VPS26 antibody

Vacuolar Protein Sorting 26 (Vacuolar Protein Sorting 26 (Yeast), VPS 26, VPS26, FLJ12930, Hbeta58, Hbeta58 Homolog, HB58, hVPS26, MGC94168, PEP8A, Vacuolar Protein Sorting 26 A, Vacuolar Protein Sorting 26A, Vacuolar Protein Sorting 26 Homolog A, Vacuola

Cat. Num. AAA6505422

• Gene Names: VPS26A; HB58; PEP8A; VPS26; Hbeta58
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Vacuolar Protein Sorting 26; Polyclonal Antibody; Vacuolar Protein Sorting 26 (Vacuolar Protein Sorting 26 (Yeast); VPS 26; VPS26; FLJ12930; Hbeta58; Hbeta58 Homolog; HB58; hVPS26; MGC94168; PEP8A; Vacuolar Protein Sorting 26 A; Vacuolar Protein Sorting 26A; Vacuolar Protein Sorting 26 Homolog A; Vacuola; anti-VPS26 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VPS26A.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-VPS26 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human VPS26A (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VPS26A Antibody (Center) in HepG2 cell line lysates (35ug/lane). VPS26A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VPS26A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(VPS26A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-VPS26 antibody

Antibodies; Protein Kinases

References

1. Haft, C.R., et.al., Mol. Biol. Cell 11(12), 4105-4116 (2000). 2. Reddy, J.V.et.al., Mol. Biol. Cell 12(10), 3242-3256 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 17978519
• NCBI GeneID: 9559
• NCBI Accession #: NP_004887.2
• NCBI GenBank Nucleotide #: NM_004896.4
• UniProt Accession #: O75436; Q8TBH4; Q9H982; A8MZ56; B2RDD3
• Molecular Weight: 28,938 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 26A isoform 1
• NCBI Official Synonym Full Names: VPS26 retromer complex component A
• NCBI Official Symbol: VPS26A
• NCBI Official Synonym Symbols: HB58; PEP8A; VPS26; Hbeta58
• NCBI Protein Information: vacuolar protein sorting-associated protein 26A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 26A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS26A
• UniProt Synonym Gene Names: VPS26; hVPS26
• UniProt Entry Name: VP26A_HUMAN

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: intracellular membrane-bound organelle; retromer complex; early endosome; endosome membrane; cytosol; endosome; vesicle

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; retrograde transport, endosome to Golgi