Goat USP15 Polyclonal Antibody | anti-USP15 antibody

Goat Polyclonal to Human USP15

Cat. Num. AAA243593

• Gene Names: USP15; UNPH4; UNPH-2
• Reactivity: Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Rabbit; Predicted Reactivity: Mouse, Rat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: USP15; Polyclonal Antibody; Goat Polyclonal to Human USP15; Anti-USP15 Antibody (C-Terminus) IHC-plus; Deubiquitinating enzyme 15; KIAA0529; Ubiquitin thioesterase 15; Ubiquitin specific protease 15; UNPH-2; UNPH4; Ubiquitin thiolesterase 15; Human USP15; anti-USP15 antibody

• Host: Goat
• Reactivity: Gibbon, Bovine, Chicken, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Rabbit; Predicted Reactivity: Mouse, Rat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human USP15.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 952

• Applicable Applications for anti-USP15 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (2.5 - 3.75 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide NDIENENCMHTN from the C-terminus of human USP15 (NP_006304.1).
• Immunogen Type: Synthetic peptide
• Immunogen: USP15 antibody was raised against synthetic peptide NDIENENCMHTN from the C-terminus of human USP15 (NP_006304.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Turkey, Chicken, Lizard, Xenopus (100%); Mouse, Rat, Opossum (92%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-USP15 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Related Product Information for anti-USP15 antibody

USP15 is an ubiquitin specific protease. Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor that once cleaved is covalently attached to other proteins by a highly specific and regulated process. USP15 can cleave the precursor form of ubiquitin and can also remove the covalently linked ubiquitin from the tagged proteins. USP15 shows widespread expression.

Product Categories/Family for anti-USP15 antibody

Family: Protease; Subfamily: Cysteine C19

NCBI and Uniprot Product Information

• NCBI GI #: 14149627
• NCBI GeneID: 9958
• NCBI Accession #: NP_006304.1
• NCBI GenBank Nucleotide #: NM_006313.2
• UniProt Accession #: Q9Y4E8; Q08AL5; Q9H8G9; Q9HCA6; Q9UNP0; Q9Y5B5
• Molecular Weight: 27,094 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 15 isoform 2
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 15
• NCBI Official Symbol: USP15
• NCBI Official Synonym Symbols: UNPH4; UNPH-2
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 15; ubiquitin thioesterase 15; deubiquitinating enzyme 15; ubiquitin thiolesterase 15; ubiquitin-specific-processing protease 15
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 15
• UniProt Gene Name: USP15
• UniProt Synonym Gene Names: KIAA0529
• UniProt Entry Name: UBP15_HUMAN

NCBI Description

This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011]

Uniprot Description

USP15: Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; transforming growth factor beta receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway