Rabbit USP11 Polyclonal Antibody | anti-USP11 antibody

USP11 Polyclonal Antibody

Cat. Num. AAA3013644

• Gene Names: USP11; UHX1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: USP11; Polyclonal Antibody; USP11 Polyclonal Antibody; UHX1; Deubiquitinating enzyme 11; Ubiquitin carboxyl-terminal hydrolase 11; Ubiquitin carboxyl-terminal hydrolase X linked; ubiquitin specific peptidase 11; Ubiquitin specific protease 11; Ubiquitin thiolesterase 11; Ubiquitin-specific-processing protease 11; UBP11; anti-USP11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USP11 polyclonal antibody detects endogenous levels of USP11 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-USP11 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:1,000; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of USP11 on 293T cell using anti-USP11 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining USP11 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-USP11 antibody

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP11 (ubiquitin specific peptidase 11), also known as UHX1, is a 920 amino acid deubiquitinating enzyme that participates in the Ub pathway. Localized to the nucleus, USP11 associates with both Ran BP-M (Ran binding protein M) and with the tumor suppressor BRCA2. Through these associations, USP11 functions to either inhibit ubiquitination of these proteins or to remove ubiquitin residues that have already been attached to these proteins. USP11 is implicated in several X-linked retinal diseases and, due to its ability to deubiquitinate BRCA2, may play a role in tumor suppression.

NCBI and Uniprot Product Information

• NCBI GI #: 24234683
• NCBI GeneID: 8237
• UniProt Accession #: P51784; Q99K46
• Molecular Weight: 110kDa
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 11
• NCBI Official Symbol: USP11
• NCBI Official Synonym Symbols: UHX1
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 11
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 11
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP11
• UniProt Synonym Gene Names: UHX1
• UniProt Entry Name: UBP11_HUMAN

NCBI Description

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23 [provided by RefSeq, Jul 2008]

Uniprot Description

USP11: Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks. Belongs to the peptidase C19 family.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system; Protease

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination