Western Blot (WB)
(KLF6 pAb tested by Western blot. 3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 ug per lane was probed with KLF6 pAb at a dilution of 2 ug/ml . Lane 1: Day 0. Lane 2: Day 10 - fully differentiated adipocytes.)
Rabbit anti-Mouse KLF6 Polyclonal Antibody | anti-KLF6 antibody
KLF6 antibody (pAb)
WB: 0.5-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(KLF6 pAb tested by Western blot. 3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 ug per lane was probed with KLF6 pAb at a dilution of 2 ug/ml . Lane 1: Day 0. Lane 2: Day 10 - fully differentiated adipocytes.)
Western Blot (WB)
(KLF6 pAb tested by Western blot. 3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 ug per lane was probed with KLF6 pAb at a dilution of 2 ug/ml . Lane 1: Day 0. Lane 2: Day 10 - fully differentiated adipocytes.)
Background: KLF6 (Kruppel-like factor 6) is member of the Kruppel-like family of transcription factors and is a zinc finger protein that acts as a transcriptional activator and functions as a tumor suppressor. Could play a role in B-cell growth and development. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis. [provided by RefSeq, May 2009]
Uniprot Description
KLF6: Transcriptional activator. Binds a GC box motif. Could play a role in B-cell growth and development. Defects in KLF6 are a cause of gastric cancer (GASC); also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. Defects in KLF6 are a cause of prostate cancer (PC). Prostate cancer is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 10p15
Cellular Component: cytoplasm; nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; B cell differentiation; cytokine and chemokine mediated signaling pathway
Disease: Gastric Cancer; Prostate Cancer
Research Articles on KLF6
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Product Notes
The KLF6 klf6 (Catalog #AAA388823) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLF6 antibody (pAb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KLF6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the KLF6 klf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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