Rabbit anti-Human, Mouse Uridine Monophosphate Kinase 1 Polyclonal Antibody | anti-MTH879 antibody

Uridine Monophosphate Kinase 1, NT (Cytidine monophosphokinase 1, UCK 1, Uridine-cytidine kinase 1, Uridine monophosphokinase 1, URK1)

Cat. Num. AAA629454

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Uridine Monophosphate Kinase 1; Polyclonal Antibody; NT (Cytidine monophosphokinase 1; UCK 1; Uridine-cytidine kinase 1; Uridine monophosphokinase 1; URK1); Anti -Uridine Monophosphate Kinase 1; anti-MTH879 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UCK. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MTH879 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human UCK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-UCK Pab is used in Western blot to detect UCK in mouse cerebellum tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-MTH879 antibody

UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.

Product Categories/Family for anti-MTH879 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 15678899
• NCBI GeneID: 1471287
• NCBI Accession #: NP_276016.1
• NCBI GenBank Nucleotide #: NC_000916.1
• UniProt Accession #: O26965
• Molecular Weight: 24,439 Da
• NCBI Official Full Name: uridine monophosphate kinase
• NCBI Official Symbol: MTH879
• NCBI Protein Information: uridine monophosphate kinase
• UniProt Protein Name: Uridylate kinase
• UniProt Gene Name: pyrH
• UniProt Synonym Gene Names: UK; UMP kinase; UMPK
• UniProt Entry Name: PYRH_METTH

Uniprot Description

Function: Catalyzes the reversible phosphorylation of UMP to UDP

By similarity. HAMAP-Rule MF_01220

Catalytic activity: ATP + UMP = ADP + UDP. HAMAP-Rule MF_01220

Enzyme regulation: Inhibited by UTP

By similarity. HAMAP-Rule MF_01220

Pathway: Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1. HAMAP-Rule MF_01220

Subunit structure: Homohexamer

By similarity. HAMAP-Rule MF_01220

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_01220.

Sequence similarities: Belongs to the UMP kinase family.