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SDS-Page

ACPP recombinant protein

ACPP, 33-386aa, Human, His tag, E Coli (Denatured)

Gene Names
ACPP; ACP3; 5'-NT; ACP-3
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
ACPP; 33-386aa; Human; His tag; E Coli (Denatured); Acid phosphatase prostate; Acid phosphatase; prostate; PAP; ACP3; ACP-3; ACPP recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MKELKFVTLV FRHGDRSPID TFPTDPIKES SWPQGFGQLT QLGMEQHYEL GEYIRKRYRK FLNESYKHEQ VYIRSTDVDR TLMSAMTNLA ALVPPEGVSI WNPILLWQPI PVHTVPLSED QLLYLPFRNC PRFQELESET LKSEEFQKRL HPYKDFIATL GKLSGLHGQD LFGIWSKVYD PLYCESVHNF TLPSRATEDT MTKLRELSEL SLLSLYGIHK QKEKSRLQGG VLVNEILNHM KRATQIPSYK KLIMYSAHDT TVSGLQMALD VYNGLLPPYA SCHLTELYFE KGEYFVEMYY RNETQHEPYP LMLPGCSPSC PLERFAELVG PVIPQDWSTE CMTTNSHQGT EDSTD
Sequence Length
386
Applicable Applications for ACPP recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for ACPP recombinant protein
ACPP, also known as Acid phosphatase, prostate. ACPP is a non-specific tyrosine phosphatase that dephosphorylates a diverse number of ubstrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. It has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Recombinant human ACPP, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for ACPP recombinant protein
References
Sharief F.S.,et al. (1992) Biochem. Biophys. Res. Commun. 184:1468-1476

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
55
Molecular Weight
43.2kDa (375aa)
NCBI Official Full Name
Acid phosphatase, prostate
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
ACPP
NCBI Official Synonym Symbols
ACP3; 5'-NT; ACP-3
NCBI Protein Information
prostatic acid phosphatase
UniProt Protein Name
Prostatic acid phosphatase
Protein Family
UniProt Gene Name
ACPP
UniProt Synonym Gene Names
PAP; 5'-NT; TMPase

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Uniprot Description

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.

Research Articles on ACPP

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Product Notes

The ACPP acpp (Catalog #AAA205924) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ACPP can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ACPP acpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MKELKFVTLV FRHGDRSPID TFPTDPIKES SWPQGFGQLT QLGMEQHYEL GEYIRKRYRK FLNESYKHEQ VYIRSTDVDR TLMSAMTNLA ALVPPEGVSI WNPILLWQPI PVHTVPLSED QLLYLPFRNC PRFQELESET LKSEEFQKRL HPYKDFIATL GKLSGLHGQD LFGIWSKVYD PLYCESVHNF TLPSRATEDT MTKLRELSEL SLLSLYGIHK QKEKSRLQGG VLVNEILNHM KRATQIPSYK KLIMYSAHDT TVSGLQMALD VYNGLLPPYA SCHLTELYFE KGEYFVEMYY RNETQHEPYP LMLPGCSPSC PLERFAELVG PVIPQDWSTE CMTTNSHQGT EDSTD. It is sometimes possible for the material contained within the vial of "ACPP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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