Rabbit UMPS Polyclonal Antibody | anti-UMPS antibody

UMPS Polyclonal Antibody

Cat. Num. AAA3011295

• Gene Names: UMPS; OPRT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UMPS; Polyclonal Antibody; UMPS Polyclonal Antibody; ODC; OMPdecase; OPRT; OPRTase; Orotate phosphoribosyl transferase and orotidine 5' decarboxylase; Orotate phosphoribosyltransferase; Orotate phosphoribosyltransferase phosphoribosyltransferase; Orotidine 5' phosphate decarboxylase; Orotidine 5''-phosphate decarboxylase; RP11-71H17.9; UMP synthase; Umps; UMPS_HUMAN; Uridine 5' monophosphate synthase; Uridine monophosphate synthetase; anti-UMPS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UMPS polyclonal antibody detects endogenous levels of UMPS protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 480

• Applicable Applications for anti-UMPS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human UMPS.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of Jurkat cells, using UMPS antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using UMPS antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using UMPS antibody at dilution of 1:200 (400x lens).)

Related Product Information for anti-UMPS antibody

Uridine 5'-monophosphate synthase (UMPS) catalyzes the last two steps of the pyrimidine biosynthetic pathway. Unlike prokaryotes, UMPS in eukaryotes combines the orotate phosphoribosyltransferase and the orotidine-5-monophosphate (OMP) decarboxylase activities into a single protein. The union of these two enzymes is thought to stabilize the catalytic centers due to the low molar concentration of the protein in mammalian cells. Loss of either enzymatic activity results in hereditary orotic aciduria, a rare autosomal recessive disorder characterized by retarded growth, anemia, and excessive urinary excretion of orotic acid. Two isoforms of UMPS exist as a result of alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 4507835
• NCBI GeneID: 7372
• NCBI Accession #: NP_000364.1
• NCBI GenBank Nucleotide #: NP_000364.1
• UniProt Accession #: P11172
• Molecular Weight: ~ 52kDa
• NCBI Official Full Name: uridine 5'-monophosphate synthase
• NCBI Official Synonym Full Names: uridine monophosphate synthetase
• NCBI Official Symbol: UMPS
• NCBI Official Synonym Symbols: OPRT
• NCBI Protein Information: uridine 5'-monophosphate synthase
• UniProt Protein Name: Uridine 5'-monophosphate synthase
• Protein Family: Uridine 5'-monophosphate synthase
• UniProt Gene Name: UMPS
• UniProt Synonym Gene Names: UMP synthase; OPRT; OPRTase; ODC
• UniProt Entry Name: UMPS_HUMAN

NCBI Description

This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

UMPS: Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1). A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.1.1.23; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; EC 2.4.2.10; Lyase

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: orotate phosphoribosyltransferase activity; orotidine-5'-phosphate decarboxylase activity

Biological Process: lactation; 'de novo' pyrimidine base biosynthetic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; UMP biosynthetic process; pyrimidine nucleoside biosynthetic process; female pregnancy

Disease: Orotic Aciduria