Human Suppression Of Tumorigenicity 14 (ST14) ELISA Kit | ST14 elisa kit

Human Suppression Of Tumorigenicity 14 (ST14) ELISA Kit

Cat. Num. AAA2020089

• Gene Names: ST14; HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
• Reactivity: Human
• Synonyms: Suppression Of Tumorigenicity 14 (ST14); Human Suppression Of Tumorigenicity 14 (ST14) ELISA Kit; SNC19; HAI; MT-SP1; PRSS14; TADG15; Epithin; Matriptase; Prostamin; Membrane-type serine protease 1; Serine protease 14; Tumor-associated differentially-expressed gene 15; ST14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Suppression Of Tumorigenicity 14 (ST14).; No significant cross-reactivity or interference between Suppression Of Tumorigenicity 14 (ST14) and analogues was observed.
• Sequence Length: 855

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.62ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppression Of Tumorigenicity 14 (ST14) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppression Of Tumorigenicity 14 (ST14) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ST14 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppression Of Tumorigenicity 14 (ST14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppression Of Tumorigenicity 14 (ST14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppression Of Tumorigenicity 14 (ST14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppression Of Tumorigenicity 14 (ST14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ST14 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119588180
• NCBI GeneID: 6768
• UniProt Accession #: Q9Y5Y6; Q9BS01; Q9H3S0; Q9HB36; Q9HCA3
• Molecular Weight: 94,770 Da
• NCBI Official Full Name: suppression of tumorigenicity 14 (colon carcinoma)
• NCBI Official Synonym Full Names: suppression of tumorigenicity 14 (colon carcinoma)
• NCBI Official Symbol: ST14
• NCBI Official Synonym Symbols: HAI; MTSP1; SNC19; ARCI11; MT-SP1; PRSS14; TADG15; TMPRSS14
• NCBI Protein Information: suppressor of tumorigenicity 14 protein
• UniProt Protein Name: Suppressor of tumorigenicity 14 protein
• Protein Family: Suppressor of tumorigenicity 14 protein
• UniProt Gene Name: ST14
• UniProt Synonym Gene Names: PRSS14; SNC19; TADG15; MT-SP1
• UniProt Entry Name: ST14_HUMAN

NCBI Description

The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

ST14: Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH). ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. Belongs to the peptidase S1 family.

Protein type: Protease; Membrane protein, integral; EC 3.4.21.109

Chromosomal Location of Human Ortholog: 11q24-q25

Cellular Component: extrinsic to plasma membrane; extracellular space; integral to plasma membrane; basolateral plasma membrane; plasma membrane

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: keratinocyte differentiation; proteolysis

Disease: Ichthyosis, Congenital, Autosomal Recessive 11