Rabbit anti-Human UMP-CMP kinase/CMPK1 Polyclonal Antibody | anti-CMPK1 antibody

Anti-UMP-CMP kinase/CMPK1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107282

• Gene Names: CMPK1; CK; CMK; UMK; CMPK; UMPK; UMP-CMPK; RP11-511I2.1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: UMP-CMP kinase/CMPK1; Polyclonal Antibody; Anti-UMP-CMP kinase/CMPK1 Antibody; Rabbit Polyclonal; CMPK1 Antibody; Rabbit PAb; Antigen Affinity Purified; cytidine monophosphate (UMP-CMP) kinase 1; cytosolic; Anti-CK Antibody; Anti-CMK Antibody; Anti-CMPK Antibody; Anti-UMK Antibody; Anti-UMP-CMPK Antibody; Anti-UMPK Antibody; anti-CMPK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CMPK1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CMPK1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human CMPK1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CMPK1 (rh CMPK1). CMPK1 specific IgG was purified by Human CMPK1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CMPK1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CMPK1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CMPK1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-CMPK1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:23 kDa)

Related Product Information for anti-CMPK1 antibody

CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells. It catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. CMPK1 also has a significant role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. In the meanwhile, CMPK1 functions in cellular nucleic acid biosynthesis.

References

Liou J, et al. (2004) Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium. Molecular Pharmacology. 67(3):806-14.Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Research. 14(10B):2121-7.Segura-Pena D, et al. (2004) Substrate-induced Conformational Changes in Human UMP/CMP Kinase. Journal of Biological Chemistry. 279(32):33882-9.

NCBI and Uniprot Product Information

• NCBI GI #: 209915597
• NCBI GeneID: 51727
• NCBI Accession #: NP_001129612.1
• NCBI GenBank Nucleotide #: NM_001136140.1
• UniProt Accession #: P30085; Q53GB7; Q5SVZ0; Q96C07; Q9UBQ8; Q9UIA2
• Molecular Weight: 22,222 Da
• NCBI Official Full Name: UMP-CMP kinase isoform b
• NCBI Official Synonym Full Names: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• NCBI Official Symbol: CMPK1
• NCBI Official Synonym Symbols: CK; CMK; UMK; CMPK; UMPK; UMP-CMPK; RP11-511I2.1
• NCBI Protein Information: UMP-CMP kinase; dCMP kinase; UMP/CMP kinase; cytidylate kinase; deoxycytidylate kinase; uridine monophosphate kinase; uridine monophosphate/cytidine monophosphate kinase
• UniProt Protein Name: UMP-CMP kinase
• UniProt Gene Name: CMPK1
• UniProt Synonym Gene Names: CMK; CMPK; UCK; UMK; UMPK; CK; dCMP kinase; UMP/CMP kinase; UMP/CMPK
• UniProt Entry Name: KCY_HUMAN

NCBI Description

This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

CMPK: Catalyzes specific phosphoryl transfer from ATP to UMP and CMP. Belongs to the adenylate kinase family.

Protein type: EC 2.7.4.14; Nucleotide Metabolism - pyrimidine; Kinase, other; EC 2.7.4.6

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: uridine kinase activity; UMP kinase activity; nucleoside diphosphate kinase activity; cytidylate kinase activity; ATP binding

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UMP biosynthetic process; phthalate metabolic process; pyrimidine ribonucleotide biosynthetic process; nucleoside diphosphate phosphorylation; UDP biosynthetic process; ovulation cycle process; 'de novo' pyrimidine base biosynthetic process; CDP biosynthetic process; dUDP biosynthetic process; nucleoside triphosphate biosynthetic process; dCDP biosynthetic process