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SDS-PAGE

CMPK1 recombinant protein

Recombinant Human CMPK1 Protein (His tag)

Gene Names
CMPK1; CK; CMK; UMK; CMPK; UMPK; UMP-CMPK
Purity
> 95 % as determined by SDS-PAGE
Synonyms
CMPK1; Recombinant Human CMPK1 Protein (His tag); CMK; CMPK; UCK; UMK; UMPK; CMPK1 recombinant protein
Ordering
For Research Use Only!
Host
Baculovirus-Insect Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Supplied as sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol
Sequence Length
179
Application Notes
The recombinant human CMPK1 consists of 214 amino acids and has a calculated molecular mass of 24.5 kDa. The recombinant protein migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
His
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for CMPK1 recombinant protein
Background: CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells. It catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. CMPK1 also has a significant role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. In the meanwhile, CMPK1 functions in cellular nucleic acid biosynthesis.

Description: A DNA sequence encoding the human CMPK1 (P30085) (Met1-Gly196) was fused with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,548 Da
NCBI Official Full Name
UMP-CMP kinase isoform b
NCBI Official Synonym Full Names
cytidine/uridine monophosphate kinase 1
NCBI Official Symbol
CMPK1
NCBI Official Synonym Symbols
CK; CMK; UMK; CMPK; UMPK; UMP-CMPK
NCBI Protein Information
UMP-CMP kinase
UniProt Protein Name
UMP-CMP kinase
UniProt Gene Name
CMPK1
UniProt Synonym Gene Names
CK; dCMP kinase; UMP/CMP kinase; UMP/CMPK

NCBI Description

This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

CMPK: Catalyzes specific phosphoryl transfer from ATP to UMP and CMP. Belongs to the adenylate kinase family.

Protein type: EC 2.7.4.14; EC 2.7.4.6; Kinase, other; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: cytoplasm; cytosol

Molecular Function: nucleoside diphosphate kinase activity; nucleoside phosphate kinase activity; uridine kinase activity

Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process; pyrimidine ribonucleotide biosynthetic process

Research Articles on CMPK1

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Product Notes

The CMPK1 cmpk1 (Catalog #AAA2545020) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CMPK1 consists of 214 amino acids and has a calculated molecular mass of 24.5 kDa. The recombinant protein migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CMPK1 cmpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CMPK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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