Rabbit UEV1/UEV1A Polyclonal Antibody | anti-UEV1/UEV1A antibody

Anti-UEV1/UEV1A Antibody

Cat. Num. AAA2401953

• Gene Names: UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• Reactivity: Human; Predicted: Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
• Purity: Affinity Purified
• Synonyms: UEV1/UEV1A; Polyclonal Antibody; Anti-UEV1/UEV1A Antibody; Rabbit Polyclonal (IgG) to Human UEV1/UEV1A; UBE2V1; CROC1; UEV1; UBE2V; UEV-1; UEV1A; CROC-1; anti-UEV1/UEV1A antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human UEV1/UEV1A.
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
• Sequence Length: 170

• Applicable Applications for anti-UEV1/UEV1A antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:10-1:100; IHC: 1:50-1:200; IHC-P: 1:50; WB: 1:500-1:2000
• Immunogen: Recombinant protein of human UBE2V1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UBE2V1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts of various cell lines.)

Related Product Information for anti-UEV1/UEV1A antibody

Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as aprotein similar in sequence and structure to the E2ubiquitin-conjugating enzymes but lacking their enzymatic activity.There are at least two variants and multiple isoforms of UEV1. Inparticular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A)has recently been shown to be an important component of the Toll-likereceptor and IL-1R signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 40806164
• NCBI GeneID: 7335
• NCBI Accession #: NP_068823.2
• NCBI GenBank Nucleotide #: NM_021988.5
• UniProt Accession #: Q13404; Q13403; Q13532; Q5TGE0; Q5TGE3; Q96H34; Q9GZT0; Q9GZW1; Q9H4J3; Q9H4J4; Q9UKL1; E1P629
• Molecular Weight: 11,766 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 variant 1 isoform a
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 V1
• NCBI Official Symbol: UBE2V1
• NCBI Official Synonym Symbols: CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 variant 1
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 variant 1
• UniProt Gene Name: UBE2V1
• UniProt Synonym Gene Names: CROC1; UBE2V; UEV1; UEV-1

NCBI Description

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Apr 2012]

Uniprot Description

Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.