Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) ELISA Kit | DYRK1A elisa kit

Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) ELISA Kit

Cat. Num. AAA2706608

• Gene Names: DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• Reactivity: Human
• Synonyms: Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A); Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) ELISA Kit; DYRK; DYRK1; HP86; MNB; MNBH; Dual specificity YAK1-related kinase; Protein kinase minibrain homolog; DYRK1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A).; No significant cross-reactivity or interference between Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) and analogues was observed.
• Sequence Length: 584

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DYRK1A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18765758
• NCBI GeneID: 1859
• NCBI Accession #: NP_001387.2
• NCBI GenBank Nucleotide #: NM_001396.4
• UniProt Accession #: Q13627; O60769; Q92582; Q92810; Q9UNM5
• Molecular Weight: 66,099 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1
• NCBI Official Synonym Full Names: dual specificity tyrosine phosphorylation regulated kinase 1A
• NCBI Official Symbol: DYRK1A
• NCBI Official Synonym Symbols: MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1A
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1A
• UniProt Synonym Gene Names: DYRK; MNB; MNBH; MNBH; hMNB

NCBI Description

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis ().