Rabbit UEV1A Polyclonal Antibody | anti-UEV1A antibody

UEV1A

Cat. Num. AAA395667

• Gene Names: UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: UEV1A; Polyclonal Antibody; anti-UEV1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as solution in phosphate buffered saline with 0.02% sodium azide
• Sequence Length: 147

• Applicable Applications for anti-UEV1A antibody: Western Blot (WB)
• Immunogen: Rabbit polyclonal UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.
• Positive Control: Postive Control: Jurkat Cell Lysate.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis of UEV1A in Jurkat cell lysates with UEV1A antibody at (A) 1, (B) 2, and (C) 4 g/ml)

Related Product Information for anti-UEV1A antibody

Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).

Product Categories/Family for anti-UEV1A antibody

Polyclonal Antibody; Signaling Intermediates

NCBI and Uniprot Product Information

• NCBI GI #: 73765546
• NCBI GeneID: 7335
• NCBI Accession #: NP_001027459.1
• NCBI GenBank Nucleotide #: NM_001032288.2
• UniProt Accession #: Q13404; Q13403; Q13532; Q5TGE0; Q5TGE3; Q96H34; Q9GZT0; Q9GZW1; Q9H4J3; Q9H4J4; Q9UKL1; E1P629
• Molecular Weight: ~15 kD
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 variant 1 isoform d
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2 variant 1
• NCBI Official Symbol: UBE2V1
• NCBI Official Synonym Symbols: CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 variant 1; DNA-binding protein; TRAF6-regulated IKK activator 1 beta Uev1A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 variant 1
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2V1
• UniProt Synonym Gene Names: CROC1; UBE2V; UEV1; UEV-1
• UniProt Entry Name: UB2V1_HUMAN

NCBI Description

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Ref.1 Ref.2 Ref.3 Ref.10 Ref.11 Ref.13 Ref.14

Subunit structure: Heterodimer with UBE2N. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with TRAF6. Ref.3

Subcellular location: Nucleus. Note: Excluded from the nucleolus. Ref.1 Ref.12

Tissue specificity: Highly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung. Ref.2 Ref.11

Induction: Down-regulated during differentiation of cultured colon adenocarcinoma cells. Ref.2

Miscellaneous: In human, TMEM189/KUA and UBE2V1/UEV1 are adjacent genes which can produce independent proteins and can also be fused to form a TMEM189-UBE2V1 hybrid protein.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution: The sequence AAH08944.2 differs from that shown. Reason: Erroneous initiation. The sequence CAC16955.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.