Rabbit UEV1A Polyclonal Antibody | anti-UBE2V1 antibody

UEV1A Antibody

Cat. Num. AAA151449

• Gene Names: UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: UEV1A Antibody is Ion exchange chromatography purified.
• Synonyms: UEV1A; Polyclonal Antibody; UEV1A Antibody; CIR1; UEV1; CROC1; UBE2V; UEV-1; CROC-1; P/OKcl.19; Ubiquitin-conjugating enzyme E2 variant 1; ubiquitin-conjugating enzyme E2 variant 1; anti-UBE2V1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Anti-UEV1A may also recognize other isoforms of UEV1A.
• Purity/Purification: UEV1A Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 170

• Applicable Applications for anti-UBE2V1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: UEV1A antibody can be used for detection of UEV1A by Western blot at 1 to 4 mug/mL.
• Conjugate: Unconjugated
• Immunogen: UEV1A antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.
• Buffer: UEV1A Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: UEV1A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of UEV1A in Jurkat cell lysates with UEV1A antibody at (A) 1, (B) 2, and (C) 4 μg/mL.)

Related Product Information for anti-UBE2V1 antibody

UEV1A Antibody: Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity. There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.

NCBI and Uniprot Product Information

• NCBI GI #: 40806167
• NCBI GeneID: 7335
• NCBI Accession #: NP_954595
• NCBI GenBank Nucleotide #: NM_199144.2
• UniProt Accession #: Q13404; Q13403; Q13532; Q5TGE0; Q5TGE3; Q96H34; Q9GZT0; Q9GZW1; Q9H4J3; Q9H4J4; Q9UKL1; E1P629
• Molecular Weight: 11,766 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 variant 1 isoform a
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2 variant 1
• NCBI Official Symbol: UBE2V1
• NCBI Official Synonym Symbols: CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 variant 1; DNA-binding protein; TRAF6-regulated IKK activator 1 beta Uev1A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 variant 1
• UniProt Gene Name: UBE2V1
• UniProt Synonym Gene Names: CROC1; UBE2V; UEV1; UEV-1
• UniProt Entry Name: UB2V1_HUMAN

NCBI Description

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Alternatively spliced transcript variants encoding multiple isoforms have been described for this gene, and multiple pseudogenes of this gene have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Apr 2012]

Uniprot Description

UBE2V1: Has no ubiquitin ligase activity on its own. The UBE2V1- UBE2N heterodimer catalyzes the synthesis of non-canonical poly- ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Belongs to the ubiquitin-conjugating enzyme family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: protein complex; ubiquitin conjugating enzyme complex; cytoplasm; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; error-free postreplication DNA repair; postreplication repair; positive regulation of transcription, DNA-dependent; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of DNA repair; regulation of transcription, DNA-dependent; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway