Western Blot (WB)
(Western Blot: Sample: Human Jurakt cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human UGGT1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Human UDP-Glucose Glycoprotein Glucosyltransferase 1 Polyclonal Antibody | anti-UGGT1 antibody
Polyclonal Antibody to UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1)
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human Jurakt cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human UGGT1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Human Jurakt cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human UGGT1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: UGGT1 knockout Jurkat cell lysate; Predicted MW: 170kd Observed MW: 170kd Primary Ab: 3ug/ml Rabbit Anti-Human UGGT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: UGGT1 knockout Jurkat cell lysate; Predicted MW: 170kd Observed MW: 170kd Primary Ab: 3ug/ml Rabbit Anti-Human UGGT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that selectively reglucosylates unfolded glycoproteins, thus providing quality control for protein transport out of the ER.[supplied by OMIM, Oct 2009]
Uniprot Description
UGCGL1: Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation. Belongs to the glycosyltransferase 8 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.4.1.-; Transferase
Chromosomal Location of Human Ortholog: 2q14.3
Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum-Golgi intermediate compartment; protein complex
Molecular Function: protein binding; UDP-glucose:glycoprotein glucosyltransferase activity; unfolded protein binding
Biological Process: de novo'posttranslational protein folding; endoplasmic reticulum unfolded protein response; protein N-linked glycosylation via asparagine
Research Articles on UGGT1
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Product Notes
The UGGT1 uggt1 (Catalog #AAA2025890) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to UDP-Glucose Glycoprotein Glucosyltransferase 1 (UGGT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UDP-Glucose Glycoprotein Glucosyltransferase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the UGGT1 uggt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UDP-Glucose Glycoprotein Glucosyltransferase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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