Rabbit anti-Human CA14 Polyclonal Antibody | anti-CA14 antibody

CA14, NT (CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV) (MaxLight 650)

Cat. Num. AAA6280207

• Gene Names: CA14; CAXiV
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CA14; Polyclonal Antibody; NT (CA14; Carbonic anhydrase 14; Carbonate dehydratase XIV; Carbonic anhydrase XIV) (MaxLight 650); Carbonic anhydrase XIV; anti-CA14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CA14 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids from the N-terminal region of human CA14.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.)

Related Product Information for anti-CA14 antibody

CA14 belongs a large family of zinc metalloenzymes that catalyze the versible hydration of carbon dioxide. The protein is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles.

Product Categories/Family for anti-CA14 antibody

Antibodies; Enzymes

References

Parkkila, S. Natl. Acad. Sci. U.S.A. 98 (4), 1918-1923 (2001). Fujikawa-Adachi, K., Nishimori, I. Genomics 61 (1), 74-81 (1999). Tarun, A.S., Bryant, B. Chem. Senses 28 (7), 621-629 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 6912284
• NCBI GeneID: 23632
• NCBI Accession #: NP_036245.1
• NCBI GenBank Nucleotide #: NM_012113.1
• UniProt Accession #: Q9ULX7; Q5TB24; Q8NCF4
• Molecular Weight: 37,668 Da
• NCBI Official Full Name: carbonic anhydrase 14
• NCBI Official Synonym Full Names: carbonic anhydrase XIV
• NCBI Official Symbol: CA14
• NCBI Official Synonym Symbols: CAXiV
• NCBI Protein Information: carbonic anhydrase 14; CA-XIV; carbonic dehydratase; carbonate dehydratase XIV
• UniProt Protein Name: Carbonic anhydrase 14
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA14
• UniProt Synonym Gene Names: CA-XIV
• UniProt Entry Name: CAH14_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; EC 4.2.1.1; Membrane protein, integral; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; metal ion binding

Biological Process: bicarbonate transport