Rabbit anti-Human, Mouse UBE2V1 Polyclonal Antibody | anti-UBE2V1 antibody

UBE2V1, CT (Ubiquitin-conjugating Enzyme E2 Variant 1, UEV-1, CROC-1, TRAF6-Regulated IKK Activator 1 beta Uev1A, CROC1, UBE2V, UEV1, P/OKcl.19)

Cat. Num. AAA630300

• Gene Names: UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: UBE2V1; Polyclonal Antibody; CT (Ubiquitin-conjugating Enzyme E2 Variant 1; UEV-1; CROC-1; TRAF6-Regulated IKK Activator 1 beta Uev1A; CROC1; UBE2V; UEV1; P/OKcl.19); Anti -UBE2V1; anti-UBE2V1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE2V1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-UBE2V1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human UBE2V1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UBE2V1 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). UBE2V1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of UBE2V1 (arrow) using rabbit polyclonal UBE2V1 Antibody (M151) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2V1 gene.)

Related Product Information for anti-UBE2V1 antibody

The CROC1 isoforms, also known as UBE2V1, show sequence similarity to ubiquitin-conjugating enzymes (UBCs, or E2s) but lack the conserved cysteine residue critical to catalytic activity of E2s.1 Northern blot analysis detected approximately 2.1- and 2.5-kb CROC1 transcripts in all human tissues examined, with the highest levels in brain, skeletal muscle, and kidney. Partial human intestinal epithelial cell cDNAs have been isolated containing the 3-primecoding sequence and 3-prime untranslated region of UBE2V1, also called UEV1.2 UEV1 does not have ubiquitin-conjugating activity in vitro. UEV1 transcripts are downregulated upon differentiation of a colon carcinoma cell line.1 Constitutive expression of exogenous UEV1 protein in these colon carcinoma cells inhibits their capacity to differentiate upon confluence and induces changes in cell cycle behavior associated with inhibition of CDK1. A heterodimeric protein complex has been identified that links TRAF6 to IKK activation.3 Peptide mass fingerprinting analysis revealed that this complex is composed of the ubiquitin conjugating enzyme UBC13 and the UBC-like protein UBE2V1, also called UEV1A. TRAF6, a RING domain protein, functions together with UBC13/UEV1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 (K63) of ubiquitin. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results unveil a new regulatory function for ubiquitin, in which IKK is activated through the assembly of K63-linked polyubiquitin chains.

Product Categories/Family for anti-UBE2V1 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 40806167
• NCBI GeneID: 7335
• NCBI Accession #: NP_954595.1
• NCBI GenBank Nucleotide #: NM_199144.1
• UniProt Accession #: Q13404; Q13403; Q13532; Q5TGE3; Q96H34; Q9GZT0; Q9GZW1; Q9H4J3; Q9H4J4; Q9UKL1; Q9UM48; E1P629; Q5TGE1
• Molecular Weight: 16,495 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 variant 1 isoform a
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2 variant 1
• NCBI Official Symbol: UBE2V1
• NCBI Official Synonym Symbols: CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 variant 1; OTTHUMP00000031795; OTTHUMP00000031796; OTTHUMP00000031801; OTTHUMP00000031805; OTTHUMP00000039980; DNA-binding protein; TRAF6-regulated IKK activator 1 beta Uev1A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 variant 1
• UniProt Gene Name: UBE2V1
• UniProt Synonym Gene Names: CROC1; UBE2V; UEV1
• UniProt Entry Name: UB2V1_HUMAN

NCBI Description

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Multiple alternatively spliced transcripts encoding different isoforms have been described for this gene. A pseudogene has been identified which is also located on chromosome 20. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Uniprot Description

UBE2V1: Has no ubiquitin ligase activity on its own. The UBE2V1- UBE2N heterodimer catalyzes the synthesis of non-canonical poly- ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Belongs to the ubiquitin-conjugating enzyme family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: protein complex; ubiquitin conjugating enzyme complex; cytoplasm; cytosol; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding

Biological Process: protein polyubiquitination; positive regulation of I-kappaB kinase/NF-kappaB cascade; error-free postreplication DNA repair; postreplication repair; positive regulation of transcription, DNA-dependent; T cell receptor signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of DNA repair; regulation of transcription, DNA-dependent; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway