Rabbit anti-Human HIST1H2BM Polyclonal Antibody | anti-HIST1H2BM antibody

HIST1H2BM Antibody (N-term)

Cat. Num. AAA9206287

• Gene Names: HIST1H2BM; H2B/e; H2BFE; dJ160A22.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HIST1H2BM; Polyclonal Antibody; HIST1H2BM Antibody (N-term); Histone H2B type 1-M; Histone H2Be; H2B/e; H2BFE; anti-HIST1H2BM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HIST1H2BM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 10-44 amino acids from the N-terminal region of human HIST1H2BM.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 126

• Applicable Applications for anti-HIST1H2BM antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from K562, PC-3 cell line and human skeletal muscle tissue lysate(from left to right), using HIST1H2BM Antibody (N-term). MBS9206287 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-HIST1H2BM antibody

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Product Categories/Family for anti-HIST1H2BM antibody

Crown Antibodies

References

Albig W.,et al.Hum. Genet. 101:284-294(1997).
Marzluff W.F.,et al.Genomics 80:487-498(2002).
Mungall A.J.,et al.Nature 425:805-811(2003).
Lubec G.,et al.Submitted (MAR-2007) to UniProtKB.
Cheung W.L.,et al.Cell 113:507-517(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4504263
• NCBI GeneID: 8342
• NCBI Accession #: NP_003512.1
• NCBI GenBank Nucleotide #: NM_003521.2
• UniProt Accession #: Q99879; Q6NWQ3
• Molecular Weight: 13989
• NCBI Official Full Name: histone H2B type 1-M
• NCBI Official Synonym Full Names: histone cluster 1, H2bm
• NCBI Official Symbol: HIST1H2BM
• NCBI Official Synonym Symbols: H2B/e; H2BFE; dJ160A22.3
• NCBI Protein Information: histone H2B type 1-M
• UniProt Protein Name: Histone H2B type 1-M
• Protein Family: Histone
• UniProt Gene Name: HIST1H2BM
• UniProt Synonym Gene Names: H2BFE; H2B/e
• UniProt Entry Name: H2B1M_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

H2B1M: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: nucleoplasm; nucleosome; nucleus

Molecular Function: DNA binding; protein heterodimerization activity

Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture