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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UBE2H antibody.)

Rabbit UBE2H Polyclonal Antibody | anti-UBE2H antibody

UBE2H Polyclonal Antibody

Gene Names
UBE2H; GID3; UBC8; UBCH; UBCH2; E2-20K
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
UBE2H; Polyclonal Antibody; UBE2H Polyclonal Antibody; GID3; UBC8; UBCH; UBCH2; E2-20K; anti-UBE2H antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Applicable Applications for anti-UBE2H antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000, IHC: 1:50-1:200
Immunogen
Recombinant protein of human UBE2H
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-131817 / sc-131819
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UBE2H antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UBE2H antibody.)
Related Product Information for anti-UBE2H antibody
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 21kDa
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 H isoform 3
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2H
NCBI Official Symbol
UBE2H
NCBI Official Synonym Symbols
GID3; UBC8; UBCH; UBCH2; E2-20K
NCBI Protein Information
ubiquitin-conjugating enzyme E2 H
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 H
UniProt Gene Name
UBE2H
UniProt Entry Name
UBE2H_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]

Uniprot Description

UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Mitochondrial; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytoplasm

Molecular Function: ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; ubiquitin-dependent protein catabolic process

Research Articles on UBE2H

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Product Notes

The UBE2H ube2h (Catalog #AAA2539572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2H Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000, IHC: 1:50-1:200. Researchers should empirically determine the suitability of the UBE2H ube2h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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