Rabbit anti-Human COPS3 Polyclonal Antibody | anti-COPS3 antibody

COPS3, ID (COP9 Signalosome Complex Subunit 3, Signalosome Subunit 3, SGN3, JAB1-containing Signalosome Subunit 3, CSN3) (AP)

Cat. Num. AAA6470744

• Gene Names: COPS3; CSN3; SGN3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: COPS3; Polyclonal Antibody; ID (COP9 Signalosome Complex Subunit 3; Signalosome Subunit 3; SGN3; JAB1-containing Signalosome Subunit 3; CSN3) (AP); anti-COPS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COPS3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
• Sequence Length: 423

• Applicable Applications for anti-COPS3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human COPS3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of COPS3 Antibody (Center) in Jurkat, 293 cell line lysates (35ug/lane). COPS3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of COPS3 (arrow) using rabbit polyclonal COPS3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COPS3 gene.)

Product Categories/Family for anti-COPS3 antibody

Antibodies; Proteins

References

1. Henriksen J., Aagesen T.H., Maelandsmo G.M., .Oncogene 22:5358-5361(2003). 2. Potocki L., Chen K.-S., Lupski J.R.Genomics 57:180-182(1999). 3. Bech-Otschir D., Kraft R., Huang X., EMBO J. 20:1630-1639(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 23238222
• NCBI GeneID: 8533
• NCBI Accession #: NP_003644
• NCBI GenBank Nucleotide #: NP_003644.2
• UniProt Accession #: Q9UNS2
• Molecular Weight: 47.873
• NCBI Official Full Name: COP9 signalosome complex subunit 3 isoform 1
• NCBI Official Synonym Full Names: COP9 signalosome subunit 3
• NCBI Official Symbol: COPS3
• NCBI Official Synonym Symbols: CSN3; SGN3
• NCBI Protein Information: COP9 signalosome complex subunit 3
• UniProt Protein Name: COP9 signalosome complex subunit 3
• UniProt Gene Name: COPS3
• UniProt Synonym Gene Names: CSN3; SGN3
• UniProt Entry Name: CSN3_HUMAN

NCBI Description

The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

COPS3: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: signalosome; nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: ubiquitin-dependent protein catabolic process; response to light stimulus; cullin deneddylation; in utero embryonic development; signal transduction