Ubiquitin-Conjugating Enzyme E2 H Recombinant Protein | UBE2H recombinant protein

Recombinant Human Ubiquitin-Conjugating Enzyme E2 H Protein

Cat. Num. AAA2556621

• Gene Names: UBE2H; GID3; UBC8; UBCH; UBCH2; E2-20K
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: Ubiquitin-Conjugating Enzyme E2 H; Recombinant Human Ubiquitin-Conjugating Enzyme E2 H Protein; UbcH2; Ubiquitin Carrier Protein H; Ubiquitin-Conjugating Enzyme E2-20K; Ubiquitin-Protein Ligase H; UBE2H; UBE2H recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5.
• Sequence Length: 113

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Met1-Leu183
• Fusion Tag: N-GST
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt.; Please minimize freeze-thaw cycles.

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Related Product Information for UBE2H recombinant protein

Protein Construction: Recombinant Human Ubiquitin-Conjugating Enzyme E2 H is produced by our E Coli expression system and the target gene encoding Met1-Leu183 is expressed with a GST tag at the N-terminus.

Background: Ubiquitin-Conjugating Enzyme E2 H (UBE2H) belongs to the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. It has been shown to conjugate ubiquitin to histone H2A in an E3 dependent manner in vitro. UBE2H is the human homolog to the yeast DNA repair gene RAD6, which is induced by DNA damaging reagents. UBE2H has been associated with cancer-induced cachexia and with the regulation of sepsis-induced muscle proteolysis.

NCBI and Uniprot Product Information

• NCBI GI #: 321267500
• NCBI GeneID: 7328
• NCBI Accession #: NP_001189427.1
• NCBI GenBank Nucleotide #: NM_001202498.1
• UniProt Accession #: P62256; P37286; Q7Z6F4; A4D1L6; C9JY93
• Molecular Weight: Molecular Mass: 47.0kDa; Actual Protein Molecular Mass: 50kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 H isoform 3
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 H
• NCBI Official Symbol: UBE2H
• NCBI Official Synonym Symbols: GID3; UBC8; UBCH; UBCH2; E2-20K
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 H
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 H
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2H

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]

Uniprot Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:8132613, PubMed:17588522, PubMed:20061386). E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex (PubMed:29911972). In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination (PubMed:20061386). Capable, in vitro, to ubiquitinate histone H2A (PubMed:8132613).