Mouse anti-Cow, Human CD62L Monoclonal Antibody | anti-CD62L antibody

Anti-Bov CD62L Alexa Fluor488

• Reactivity: Cow, Human
• Applications: Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD62L; Monoclonal Antibody; Anti-Bov CD62L Alexa Fluor488; L-selectin; LSEL; SELL; LAM1; LECAM1; LEU8; LNHR; LYAM1; PLNHR; selectin L; anti-CD62L antibody

• Host: Mouse
• Reactivity: Cow, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: IVA94
• Specificity: The antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD62L antibody: Immunocytochemistry (ICC), Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-5ug/ml
• Conjugation: Alexa Fluor488
• Immunogen: Bovine leukocytes.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) Alexa Fluor 488 antibody (concentration in sample 1 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-CD62L (IVA94) Alexa Fluor 488 antibody (concentration in sample 5 ug/ml).Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-CD62L (IVA94) Alexa Fluor 488 antibody (concentration in sample 5 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of bovine CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) Alexa Fluor 488 antibody (concentration in sample 1 ug/ml).)

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-CD62L (IVA94) Alexa Fluor 488 antibody (concentration in sample 5 ug/ml).)

Related Product Information for anti-CD62L antibody

CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).

References

*Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076dopt=A,*Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996)., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204dopt=A,*Naessens J, Nthale JM, Muiya P: Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug;52(4):347-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896224dopt=A,*Howard CJ: Ruminant cluster CD62L. Vet Immunol Immunopathol. 1996 Aug;52(4):255-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896209dopt=A

NCBI and Uniprot Product Information

• NCBI GI #: 27901801
• NCBI GeneID: 281485
• NCBI Accession #: NP_776607.1
• NCBI GenBank Nucleotide #: NM_174182.1
• UniProt Accession #: P98131
• Molecular Weight: 41,971 Da
• NCBI Official Full Name: L-selectin
• NCBI Official Symbol: SELL
• NCBI Protein Information: L-selectin; CD62 antigen-like family member L; LAM-1; LECAM1; leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 1; lymph node homing receptor; lymphocyte adhesion molecule 1; selectin L (lymphocyte adhesion molecule 1)
• UniProt Protein Name: L-selectin
• UniProt Gene Name: SELL
• UniProt Synonym Gene Names: LAM-1; LECAM1
• UniProt Entry Name: LYAM1_BOVIN

Uniprot Description

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia ().