Rabbit anti-Human Ube2H Polyclonal Antibody | anti-UBE2H antibody

Anti-Ube2H Antibody, Rabbit Polyclonal

Cat. Num. AAA8106619

• Gene Names: UBE2H; GID3; UBC8; UBCH; UBCH2; E2-20K
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Ube2H; Polyclonal Antibody; Anti-Ube2H Antibody; Rabbit Polyclonal; UBE2H Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquitin-conjugating enzyme E2H; Anti-E2-20K Antibody; Anti-GID3 Antibody; Anti-UBC8 Antibody; Anti-UBCH Antibody; Anti-UBCH2 Antibody; anti-UBE2H antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UBE2H
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UBE2H antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human UBE2H protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human UBE2H (rh UBE2H). UBE2H specific IgG was purified by Human UBE2H affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(UBE2H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-UBE2H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UBE2H rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-UBE2H rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:21 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-UBE2H antibody

UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by a ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin-protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.

References

Sjoerd J L van Wijk, et al. (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol Syst Biol. 5: 317.Nandi D, et al. (2006) The ubiquitin-proteasome system. Journal of biosciences. 31 (1): 137-55.

NCBI and Uniprot Product Information

• NCBI GI #: 51338683
• NCBI GeneID: 7328
• UniProt Accession #: P62256; P37286; Q7Z6F4; A4D1L6; C9JY93
• Molecular Weight: 183
• NCBI Official Full Name: Ubiquitin-conjugating enzyme E2 H
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2H
• NCBI Official Symbol: UBE2H
• NCBI Official Synonym Symbols: GID3; UBC8; UBCH; UBCH2; E2-20K
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 H; ubiquitin-protein ligase H; ubiquitin carrier protein H; GID complex subunit 3, UBC8 homolog; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 H
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2H
• UniProt Entry Name: UBE2H_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]

Uniprot Description

UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Mitochondrial; Ligase

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: cytoplasm

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process