Rabbit anti-Mouse Fibronectin Polyclonal Antibody | anti-Fn1 antibody

Fibronectin (PE)

Cat. Num. AAA6476409

• Gene Names: Fn1; Fn; Fn-1; E330027I09
• Reactivity: Mouse
• Applications: ELISA, Immunohistochemistry, immunoassay, Radioimmunoassay, Immunofluorescence, Immunocytochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Fibronectin; Polyclonal Antibody; Fibronectin (PE); anti-Fn1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse fibronectin. Less than 0.1% reactivity with mouse Laminin and Collagen Types I, III and IV by radioimmunoassay.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Fn1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Radioimmunoassay (RIA), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:200; IHC: 1:80 for immunofluorescent staining of frozen mouse skin and liver tissues.; Applications are based on unconjugated antibody.
• Immunogen: Fibronectin extracted and purified from mouse plasma.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunocytochemistry (ICC)

(Immunocytochemistry: Confocal fluorescent analysis of NIH/3T3 using F4215-46 (Red). Actin filaments have been labeled with AlexaFluor 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive staining is restricted to the cytosol.)

Product Categories/Family for anti-Fn1 antibody

Antibodies; Fibronectin

References

Hanke, M.L. et al., (2012) PLoS ONE 7(8): e42476. doi:10.1371/journal.pone.0042476. 1. Knuesel, I., et al., Neurobiol Aging. 30(5): 697-716 (2009). 2. Murase, S. and Horwitz, A.F., J. Neurosci. 22(9): 3568-3579 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 46849812
• NCBI GeneID: 14268
• NCBI Accession #: NP_034363.1
• NCBI GenBank Nucleotide #: NM_010233.2
• UniProt Accession #: P11276; Q61567; Q61568; Q61569; Q64233; Q80UI4; G5E8B8
• Molecular Weight: 160,000
• NCBI Official Full Name: fibronectin isoform a
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: Fn; Fn-1; E330027I09
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_MOUSE

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrinogen complex; apical plasma membrane; ER-Golgi intermediate compartment; extracellular region; basement membrane; basal lamina

Molecular Function: integrin binding; heparin binding; protein binding; protease activator activity; protease binding; mercury ion binding

Biological Process: integrin activation; extracellular matrix organization and biogenesis; wound healing; positive regulation of chemotaxis; cell-matrix adhesion; positive regulation of axon extension; glial cell migration; cell activation; regulation of cell shape; cell-substrate junction assembly; acute-phase response; calcium-independent cell-matrix adhesion; peptide cross-linking; angiogenesis; cell adhesion; positive regulation of cell migration; negative regulation of apoptosis