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Western Blot (WB) (UBE2F antibody (MBS536478) used at 0.2-1 ug/ml to detect target protein.)

Rabbit UBE2F Polyclonal Antibody | anti-UBE2F antibody

UBE2F antibody

Gene Names
UBE2F; NCE2
Applications
Western Blot
Synonyms
UBE2F; Polyclonal Antibody; UBE2F antibody; Polyclonal UBE2F; Anti-UBE2F; NCE2; UBEF 2; UBEF-2; ubiquitin-conjugating enzyme E2F; MGC18120; putative; anti-UBE2F antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Form/Format
Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration
1 mg/ml (varies by lot)
Sequence Length
185
Applicable Applications for anti-UBE2F antibody
Western Blot (WB)
Application Notes
WB: 0.2-1 ug/ml
Biological Significance
UBE2F belongs to the ubiquitin-conjugating enzyme family. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
Cross-Reactivity
Human
Immunogen
UBE2F antibody was raised in rabbit using the middle region of UBE2F as the immunogen
Preparation and Storage
Store at 4 degree C, following reconstitution, aliquot and store at -20 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(UBE2F antibody (MBS536478) used at 0.2-1 ug/ml to detect target protein.)

Western Blot (WB) (UBE2F antibody (MBS536478) used at 0.2-1 ug/ml to detect target protein.)
Related Product Information for anti-UBE2F antibody
Affinity purified rabbit polyclonal UBE2F antibody
Product Categories/Family for anti-UBE2F antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,158 Da
NCBI Official Full Name
NEDD8-conjugating enzyme UBE2F isoform 1
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
Protein Family
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
UniProt Entry Name
UBE2F_HUMAN

Uniprot Description

UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function: protein binding; ubiquitin protein ligase binding; ATP binding; NEDD8 ligase activity; ligase activity

Biological Process: protein neddylation; protein ubiquitination

Research Articles on UBE2F

Similar Products

Product Notes

The UBE2F ube2f (Catalog #AAA536478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's UBE2F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.2-1 ug/ml. Researchers should empirically determine the suitability of the UBE2F ube2f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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