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Western Blot (WB) (Western blot analysis of extracts from K562 cells and A549 cells, using DAK antibody. )

Rabbit DAK Polyclonal Antibody | anti-DAK antibody

DAK Antibody

Gene Names
DAK; NET45
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DAK; Polyclonal Antibody; DAK Antibody; Dihydroxyacetone kinase; EC 2.7.1.29; Glycerone kinase; DHA kinase; anti-DAK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Applicable Applications for anti-DAK antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:3000
Immunogen
Synthesized peptide derived from internal of human DAK.
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from K562 cells and A549 cells, using DAK antibody. )

Western Blot (WB) (Western blot analysis of extracts from K562 cells and A549 cells, using DAK antibody. )
Product Categories/Family for anti-DAK antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,947 Da
NCBI Official Full Name
bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
NCBI Official Synonym Full Names
dihydroxyacetone kinase 2 homolog (S. cerevisiae)
NCBI Official Symbol
DAK
NCBI Official Synonym Symbols
NET45
NCBI Protein Information
bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing); DHA kinase; glycerone kinase; Dha kinase/FMN cyclase; FAD-AMP lyase cyclizing; FAD-AMP lyase cyclic
UniProt Protein Name
Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
Protein Family
UniProt Gene Name
DAK
UniProt Synonym Gene Names
DHA kinase
UniProt Entry Name
DHAK_HUMAN

NCBI Description

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DAK: Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Belongs to the dihydroxyacetone kinase (DAK) family.

Protein type: EC 2.7.1.29; Lipid Metabolism - glycerolipid; Kinase, other; EC 4.6.1.15; EC 2.7.1.28

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleus; cytosol

Molecular Function: metal ion binding; FAD-AMP lyase (cyclizing) activity; triokinase activity; ATP binding; glycerone kinase activity

Biological Process: glycerol metabolic process; carbohydrate phosphorylation; innate immune response; cellular carbohydrate metabolic process; regulation of innate immune response

Research Articles on DAK

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Product Notes

The DAK dak (Catalog #AAA7130885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAK Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DAK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:3000. Researchers should empirically determine the suitability of the DAK dak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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