Myosin IE (MYO1E) Recombinant Protein | MYO1E recombinant protein

Recombinant Myosin IE (MYO1E)

Cat. Num. AAA2009736

• Gene Names: MYO1E; FSGS6; MYO1C; HuncM-IC
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myosin IE (MYO1E); Recombinant Myosin IE (MYO1E); MYO1E recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-KRDLLLTPKC LYLIGREKVK QGPDKGLVKE VLKRKIEIER ILSVSLSTMQ DDIFILHEQE YDSLLESVFK TEFLSLLAKR YEEKTQKQLP LKFSNTLELK LKKENWGPWS AGGSRQVQFH QGFGDLAVLK PSNKVLQVSI GPGLPKNSRP TRRNTTQNTG YSSGTQNANY PVRAAPPPPG YHQNGVIRNQ YVPYPHAPGS QRSNQKSLYT SMARPPLPRQ QSTSSDRVSQ TPESLDFLKV PDQGAAGVRR QTTSRP
• Sequence Length: 1108

• Applicable Applications for MYO1E recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Lys781~Pro1036 (Accession # Q12965) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 55956916
• NCBI GeneID: 4643
• NCBI Accession #: NP_004989.2
• NCBI GenBank Nucleotide #: NM_004998.3
• UniProt Accession #: Q12965; Q14778
• Molecular Weight: 30.1kDa
• NCBI Official Full Name: unconventional myosin-Ie
• NCBI Official Synonym Full Names: myosin IE
• NCBI Official Symbol: MYO1E
• NCBI Official Synonym Symbols: FSGS6; MYO1C; HuncM-IC
• NCBI Protein Information: unconventional myosin-Ie; myosin-IC; MYO1E variant protein; unconventional myosin 1E
• UniProt Protein Name: Unconventional myosin-Ie
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO1E
• UniProt Synonym Gene Names: MYO1C
• UniProt Entry Name: MYO1E_HUMAN

NCBI Description

This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012]

Uniprot Description

MYO1E: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14. Defects in MYO1E are the cause of focal segmental glomerulosclerosis type 6 (FSGS6). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. FSGS6 is a childhood-onset disorder resulting in nephrotic syndrome, which includes massive proteinuria, hypoalbuminemia, hyperlipidemia, and edema.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 15q21-q22

Cellular Component: adherens junction; cytoskeleton; cytoplasm; clathrin-coated endocytic vesicle; intercellular junction; myosin complex; actin cytoskeleton

Molecular Function: microfilament motor activity; calmodulin binding; actin filament binding; protein binding; motor activity; ATPase activity, coupled; ATP binding; phosphoinositide binding

Biological Process: nitrogen compound metabolic process; actin filament-based movement; post-embryonic hemopoiesis; in utero embryonic development; glomerular basement membrane development; glomerular filtration; endocytosis; platelet-derived growth factor receptor signaling pathway; vasculogenesis

Disease: Focal Segmental Glomerulosclerosis 6