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Western Blot (WB) (Western Blot analysis of K562 cells using RHOBTB3 Polyclonal Antibody)

Rabbit anti-Human, Mouse RHOBTB3 Polyclonal Antibody | anti-RHOBTB3 antibody

RHOBTB3 Polyclonal Antibody

Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
RHOBTB3; Polyclonal Antibody; RHOBTB3 Polyclonal Antibody; KIAA0878; Rho-related BTB domain-containing protein 3; anti-RHOBTB3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
RHOBTB3 Polyclonal Antibody detects endogenous levels of RHOBTB3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
611
Applicable Applications for anti-RHOBTB3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
Immunocytochemistry: 1:200 - 1:1000
ELISA: 1:40000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the Internal region of human RHOBTB3.
Target Name
RHOBTB3
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of K562 cells using RHOBTB3 Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of K562 cells using RHOBTB3 Polyclonal Antibody)
Product Categories/Family for anti-RHOBTB3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,413 Da
NCBI Official Full Name
rho-related BTB domain-containing protein 3
NCBI Official Synonym Full Names
Rho-related BTB domain containing 3
NCBI Official Symbol
RHOBTB3
NCBI Protein Information
rho-related BTB domain-containing protein 3
UniProt Protein Name
Rho-related BTB domain-containing protein 3
UniProt Gene Name
RHOBTB3
UniProt Synonym Gene Names
KIAA0878
UniProt Entry Name
RHBT3_HUMAN

NCBI Description

RHOBTB3 is a member of the evolutionarily conserved RHOBTB subfamily of Rho GTPases. For background information on RHOBTBs, see RHOBTB1 (MIM 607351).[supplied by OMIM, Apr 2004]

Uniprot Description

RHOBTB3: Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

Protein type: EC 3.6.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: Golgi apparatus

Molecular Function: protein binding; GTP binding; ATPase activity; ATP binding; Rab GTPase binding

Biological Process: metabolic process; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi

Research Articles on RHOBTB3

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Product Notes

The RHOBTB3 rhobtb3 (Catalog #AAA9404766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHOBTB3 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RHOBTB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunocytochemistry: 1:200 - 1:1000 ELISA: 1:40000 Not yet tested in other applications. Researchers should empirically determine the suitability of the RHOBTB3 rhobtb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHOBTB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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