Rabbit UBA5 Polyclonal Antibody | anti-UBA5 antibody

UBA5 Polyclonal Antibody

Cat. Num. AAA3007592

• Gene Names: UBA5; EIEE44; SCAR24; THIFP1; UBE1DC1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBA5; Polyclonal Antibody; UBA5 Polyclonal Antibody; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1; UBE1DC1; anti-UBA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBA5 polyclonal antibody detects endogenous levels of UBA5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 404

• Applicable Applications for anti-UBA5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human UBA5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBA5 polyclonal antibody at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:HCT116 whole cell lysate (40ug))

Related Product Information for anti-UBA5 antibody

Uba5 is the smallest ubiquitin-like molecule-activating enzyme and contains an adenylation domain and a C-terminal region. This enzyme only exists in multicellular organisms. Diseases associated with UBA5 include Epileptic Encephalopathy, Early Infantile, 44 and Spinocerebellar Ataxia, Autosomal Recessive, 24.

NCBI and Uniprot Product Information

• NCBI GI #: 13376212
• NCBI GeneID: 79876
• NCBI Accession #: NP_079094.1
• NCBI GenBank Nucleotide #: NP_079094.1
• UniProt Accession #: Q9GZZ9
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin like modifier activating enzyme 5
• NCBI Official Symbol: UBA5
• NCBI Official Synonym Symbols: EIEE44; SCAR24; THIFP1; UBE1DC1
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 5
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 5
• UniProt Gene Name: UBA5
• UniProt Synonym Gene Names: UBE1DC1; Ubiquitin-activating enzyme 5
• UniProt Entry Name: UBA5_HUMAN

NCBI Description

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1. [provided by RefSeq, Feb 2016]

Uniprot Description

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway