Apolipoprotein A4 (APOA4) Recombinant Protein | APOA4 recombinant protein

Recombinant Apolipoprotein A4 (APOA4)

Cat. Num. AAA2010005

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apolipoprotein A4 (APOA4); Recombinant Apolipoprotein A4 (APOA4); APOA4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-EVNADQVATV MWDYFSQLGS NAKKAVEHLQ KSELTQQLNT LFQDKLGEVN TYTEDLQKKL VPFATELHER LTKDSEKLKE EIRRELEELR ARLLPHATEVSQKIGDNVRE LQQRLGPFTG GLRTQVNTQV QQLQRQLKPY AERMESVLRQ NIRNLEASVA PYADEFKAKI DQNVEELKGS LTPYAEELKA KIDQNVEELR RSLAPYAQDV QEKLNHQLEG LAFQMKKQAE ELKAKISANA DELRQKLVPV AENVHGHLKG NTEGLQKSLL ELRSHLDQQV EEFRLKVEPY GETFNKALVQ QVEDLRQKLG PLAGDVEGHL SFLEKDLRDK VNTFFSTLKEEASQGQSQAL PAQEKAQAPL EG
• Sequence Length: 382

• Applicable Applications for APOA4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Glu21~Gly382 (Accession # O46409) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47523830
• NCBI GeneID: 397681
• NCBI Accession #: NP_999553.1
• NCBI GenBank Nucleotide #: NM_214388.1
• UniProt Accession #: O46409; A9LM22
• Molecular Weight: 42.8kDa
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_PIG

Uniprot Description

Function: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

Subunit structure: Homodimer

By similarity.

Subcellular location: Secreted.

Tissue specificity: Secreted in plasma.

Domain: Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.