Rabbit anti-Human Tyrosyl tRNA synthetase Polyclonal Antibody | anti-TYRRS antibody

Tyrosyl tRNA synthetase, CT (YARS, CMTDIC, TYRRS, YRS, YTS) (Biotin)

Cat. Num. AAA6504311

• Gene Names: YARS; YRS; YTS; TYRRS; CMTDIC
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Tyrosyl tRNA synthetase; Polyclonal Antibody; CT (YARS; CMTDIC; TYRRS; YRS; YTS) (Biotin); anti-TYRRS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human YARS.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TYRRS antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human YARS (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-YARS Antibody (C-term) in CEM cell line lysates (35ug/lane). YARS(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with YARS antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-TYRRS antibody

Antibodies; Enzymes; Synthetase

References

1. Yang, X.L., Chem. Biol. 14(12), 1323-1333 (2007). 2. Jordanova, A., Nat. Genet. 38(2), 197-202 (2006). 3. Bonnefond, L., Biochemistry 44(12), 4805-4816 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4507947
• NCBI GeneID: 8565
• NCBI Accession #: NP_003671
• NCBI GenBank Nucleotide #: NM_003680.3
• UniProt Accession #: P54577; O43276; Q53EN1; B3KWK4; D3DPQ4
• Molecular Weight: 61.3 kDa (548aa)
• NCBI Official Full Name: tyrosine--tRNA ligase, cytoplasmic
• NCBI Official Synonym Full Names: tyrosyl-tRNA synthetase
• NCBI Official Symbol: YARS
• NCBI Official Synonym Symbols: YRS; YTS; TYRRS; CMTDIC
• NCBI Protein Information: tyrosine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Tyrosine--tRNA ligase, cytoplasmic
• UniProt Gene Name: YARS
• UniProt Synonym Gene Names: TyrRS
• UniProt Entry Name: SYYC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.1; Ligase

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: extracellular space; cytoplasm; cytosol; nucleus

Molecular Function: signal transducer activity; tyrosine-tRNA ligase activity; ATP binding; tRNA binding; isoleucine-tRNA ligase activity; interleukin-8 receptor binding

Biological Process: isoleucyl-tRNA aminoacylation; tyrosyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; apoptosis; gene expression; signal transduction

Disease: Charcot-marie-tooth Disease, Dominant Intermediate C