Rabbit anti-Human Tyrosyl tRNA synthetase Polyclonal Antibody | anti-TYRRS antibody

Tyrosyl tRNA synthetase, CT (YARS, CMTDIC, TYRRS, YRS, YTS) (FITC)

Cat. Num. AAA6504312

• Gene Names: YARS; YRS; YTS; TYRRS; CMTDIC
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Tyrosyl tRNA synthetase; Polyclonal Antibody; CT (YARS; CMTDIC; TYRRS; YRS; YTS) (FITC); anti-TYRRS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human YARS.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TYRRS antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human YARS (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of anti-YARS Antibody (C-term) in CEM cell line lysates (35ug/lane). YARS(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with YARS antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-TYRRS antibody

Antibodies; Enzymes; Synthetase

References

1. Yang, X.L., Chem. Biol. 14(12), 1323-1333 (2007). 2. Jordanova, A., Nat. Genet. 38(2), 197-202 (2006). 3. Bonnefond, L., Biochemistry 44(12), 4805-4816 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4507947
• NCBI GeneID: 8565
• NCBI Accession #: NP_003671
• NCBI GenBank Nucleotide #: NM_003680.3
• UniProt Accession #: P54577; O43276; Q53EN1; B3KWK4; D3DPQ4
• Molecular Weight: 61.3 kDa (548aa)
• NCBI Official Full Name: tyrosine--tRNA ligase, cytoplasmic
• NCBI Official Synonym Full Names: tyrosyl-tRNA synthetase
• NCBI Official Symbol: YARS
• NCBI Official Synonym Symbols: YRS; YTS; TYRRS; CMTDIC
• NCBI Protein Information: tyrosine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Tyrosine--tRNA ligase, cytoplasmic
• UniProt Gene Name: YARS
• UniProt Synonym Gene Names: TyrRS
• UniProt Entry Name: SYYC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.1; Ligase

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: extracellular space; cytoplasm; cytosol; nucleus

Molecular Function: signal transducer activity; tyrosine-tRNA ligase activity; ATP binding; tRNA binding; isoleucine-tRNA ligase activity; interleukin-8 receptor binding

Biological Process: isoleucyl-tRNA aminoacylation; tyrosyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; apoptosis; gene expression; signal transduction

Disease: Charcot-marie-tooth Disease, Dominant Intermediate C