Rabbit anti-Mouse, Rat Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Tyrosine Hydroxylase, phosphorylated (Ser31) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Cat. Num. AAA630694

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; phosphorylated (Ser31) (TH; TYH; Tyrosine 3 Hydroxylase; Tyrosine 3 Monooxygenase); Anti -Tyrosine Hydroxylase; anti-TH antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Tyrosine Hydroxylase, phosphorylated at Ser31. Specific for the 62kD TH in Western Blots of PC-12 cell lysates.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-TH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western Blot: 1:1000, Immunofluorescence: 1:1000 (frozen sections), Immunohistochemistry: 1:1000 (frozen sections), Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding Ser31 of rat TH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Phospho-Tyrosine Hydroxylase (S31) by Western Blot)

Related Product Information for anti-TH antibody

Rat tyrosine hydroxylase (TH) is a pterin-dependent monooxygenase that catalyzes the hydroxylation of tyrosine to DOPA through the use of a non-heme iron. It is a 55 kD, 498 amino acid (aa) -helical protein that runs anomalously at 62 kD in SDS-PAGE. The molecule contains a 164 aa N-terminal regulatory region and a 334 aa C-terminal catalytic domain. Its activity is regulated at the post-transcriptional level by phosphorylation of serine and feedback by catecholamines. Four serines are known to be phosphorylated at aa positions 8, 19, 31, and 40. Different kinases contribute to different phosphorylation patterns. For example, MAPKAPK-2 and CaMKII act on S19 and S40, PKA phosphorylates only at S40, while Cdk5 phosphorylates S31. Variable site phosphorylations have variable effects. S40 phosphorylation blocks catecholamine feedback inhibition. Subsequent phosphorylations at S19 or S31 likely stabilize an otherwise unstable enzyme phosphorylated only at S40, contributing to increased enzyme activity. Phosphorylation at S8 is likely to be physiologically unimportant.

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 986909
• NCBI GeneID: 7054
• UniProt Accession #: O43816; P07101; P78428; Q0PWM2; Q0PWM3; Q15587; Q16846; Q2M3B4; Q6XS77; Q7KZ36
• Molecular Weight: 14,058 Da
• NCBI Official Full Name: tyrosine hydroxylase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; OTTHUMP00000011163; OTTHUMP00000011225; OTTHUMP00000011226; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine hydroxylase
• UniProt Entry Name: O43816_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Vesicle; Mitochondrial; EC 1.14.16.2; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; cytoplasm; melanosome membrane; dendrite; terminal button; perikaryon; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to pyrethroid; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; response to peptide hormone stimulus; synaptic transmission, dopaminergic; locomotory behavior; fatty acid metabolic process; regulation of heart contraction; response to estradiol stimulus; response to salt stress; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; memory; synaptic vesicle amine transport; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive