Rabbit anti-Human Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Tyrosine Hydroxylase, phosphorylated (Ser40) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Cat. Num. AAA625642

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; phosphorylated (Ser40) (TH; TYH; Tyrosine 3 Hydroxylase; Tyrosine 3 Monooxygenase); Anti -Tyrosine Hydroxylase; anti-TH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-TH antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunocytochemistry., , Recommended Dilution:, Western Blot: 1:1000, Immunocytochemistry (IF): 1:200, Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic phosphopeptide (KLH coupled) around Ser40 of human tyrosine hydroxylase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #T9237-10A (lower).)

Testing Data

(Confocal images of brain cortical secions from rats, mock-treated (control) or subjected to 15 minutes ischemia followed by 24 hours of reperfusion, using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (green) and propidium iodide (red). Phospho-tyrosine hydrolase is decreased during 15 minutes ischemia and returns to control levels after 24 hours of reperfusion. Images provided by Dr. Bingren Hu, University of Miami, School of Medicine, Miami, Florida.)

Immunofluorescence (IF)

(Immunofluorescent staining of rat PC12 cells, untreated (A-C) or forskolin-treated (D-F), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (A, D), the same antibody preincubated with either the phospho-antigenic peptide (B, E) the corresponding nonphospho-peptide (C, F).)

Related Product Information for anti-TH antibody

Tyrosine hydroxylase catalyzes the rate limiting step in the synthesis of dopamine and other catecholamines. The enzyme's activity is regulated at the levels of transcription, translation and post-translational modification. Tyrosine hydroxylase exists in several different isoforms ranging from 50kD to about 62kD in human. The N-terminal regulatory domain contains three phospho-serine sites: Ser19, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 986909
• NCBI GeneID: 7054
• UniProt Accession #: O43816; P07101; P78428; Q0PWM2; Q0PWM3; Q15587; Q16846; Q2M3B4; Q6XS77; Q7KZ36
• Molecular Weight: 14,058 Da
• NCBI Official Full Name: tyrosine hydroxylase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; OTTHUMP00000011163; OTTHUMP00000011225; OTTHUMP00000011226; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine hydroxylase
• UniProt Entry Name: O43816_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; EC 1.14.16.2; Mitochondrial; Endoplasmic reticulum; Vesicle; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; cytoplasm; melanosome membrane; dendrite; terminal button; perikaryon; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; synaptic transmission, dopaminergic; response to peptide hormone stimulus; heart development; response to lipopolysaccharide; locomotory behavior; dopamine biosynthetic process; fatty acid metabolic process; regulation of heart contraction; response to salt stress; phytoalexin metabolic process; response to estradiol stimulus; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; sensory perception of sound; visual perception; response to electrical stimulus; epinephrine biosynthetic process; glycoside metabolic process; response to corticosterone stimulus; response to pyrethroid; neurotransmitter biosynthetic process; response to nutrient levels; response to light stimulus; terpene metabolic process; anatomical structure morphogenesis; sphingolipid metabolic process; eating behavior; response to amphetamine; phthalate metabolic process; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; social behavior; response to herbicide; response to ether; memory; synaptic vesicle amine transport; eye photoreceptor cell development; organ morphogenesis; response to ethanol; circadian sleep/wake cycle; pigmentation; response to zinc ion; response to hypoxia; response to activity; cerebral cortex development; embryonic camera-type eye morphogenesis; response to water deprivation

Disease: Segawa Syndrome, Autosomal Recessive