Rabbit anti-Rat Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Tyrosine Hydroxylase, phosphorylated (Ser31) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Cat. Num. AAA624954

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified; Supplied as a liquid in sequential chromatography on phospho- and nonphospho- peptide affinity columns
• Synonyms: Tyrosine Hydroxylase; Polyclonal Antibody; phosphorylated (Ser31) (TH; TYH; Tyrosine 3 Hydroxylase; Tyrosine 3 Monooxygenase); Anti -Tyrosine Hydroxylase; anti-TH antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for rat TH phosphorylated at Ser31. On Western blots, it identifies a single band at ~60kD. Reactivity has been confirmed with PC-12 cell lysates stimulated by okadaic acid.
• Purity/Purification: Purified; Supplied as a liquid in sequential chromatography on phospho- and nonphospho- peptide affinity columns
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM NaCl, 0.1mg/ml BSA and 50% glycerol.

• Applicable Applications for anti-TH antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunofluorescence: 1:1000; Immunohistochemistry: 1:1000
• Immunogen: Synthetic phospho peptide derived from a region of the rat tyrosine hydroxylase (TH) protein surrounding the phosphorylated Ser31 residue.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TH antibody

Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines dopamine and norepinephrine from tyrosine. TH is therefore a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. Phosphorylation of Ser31 can activate TH in vitro, and has been observed at this residue following NGF, phorbol ester, and diacylglycerol treatment of PC12 cells. Ser31 has been identified as a membrane depolarization-dependent and growth factor-sensitive phosphorylation site.

Product Categories/Family for anti-TH antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 986909
• NCBI GeneID: 7054
• UniProt Accession #: O43816; P07101; P78428; Q0PWM2; Q0PWM3; Q15587; Q16846; Q2M3B4; Q6XS77; Q7KZ36
• Molecular Weight: 14,058 Da
• NCBI Official Full Name: tyrosine hydroxylase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; OTTHUMP00000011163; OTTHUMP00000011225; OTTHUMP00000011226; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine hydroxylase
• UniProt Entry Name: O43816_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Mitochondrial; Oxidoreductase; Vesicle; Endoplasmic reticulum; EC 1.14.16.2; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; cytoplasm; dendrite; melanosome membrane; terminal button; perikaryon; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; synaptic transmission, dopaminergic; response to peptide hormone stimulus; heart development; response to lipopolysaccharide; locomotory behavior; dopamine biosynthetic process; fatty acid metabolic process; regulation of heart contraction; phytoalexin metabolic process; response to salt stress; response to estradiol stimulus; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; sensory perception of sound; visual perception; response to electrical stimulus; glycoside metabolic process; epinephrine biosynthetic process; response to pyrethroid; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to nutrient levels; response to light stimulus; anatomical structure morphogenesis; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; phthalate metabolic process; mating behavior; multicellular organismal aging; isoquinoline alkaloid metabolic process; social behavior; response to herbicide; learning; response to ether; synaptic vesicle amine transport; memory; eye photoreceptor cell development; organ morphogenesis; circadian sleep/wake cycle; response to ethanol; pigmentation; response to zinc ion; response to hypoxia; embryonic camera-type eye morphogenesis; response to activity; cerebral cortex development; response to water deprivation

Disease: Segawa Syndrome, Autosomal Recessive