Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit | VLDLR elisa kit

Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit

Cat. Num. AAA261206

• Reactivity: Rat
• Synonyms: Very Low Density Lipoprotein Receptor (VLDLR); Rat Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit; VLDLR elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 873

• Samples: Rat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Vglut1 Concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VLDLR elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat VGluT1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 688371
• Molecular Weight: 93,383 Da
• NCBI Official Full Name: very low density lipoprotein receptor
• UniProt Protein Name: Very low-density lipoprotein receptor
• Protein Family: Very low-density lipoprotein receptor
• UniProt Gene Name: VLDLR
• UniProt Synonym Gene Names: VLDL receptor; VLDL-R
• UniProt Entry Name: VLDLR_HUMAN

Uniprot Description

VLDLR: Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1); also known as dysequilibrium syndrome (DES) or non- progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: membrane; integral to membrane; plasma membrane; coated pit; receptor complex

Molecular Function: low-density lipoprotein receptor activity; very-low-density lipoprotein receptor activity; very-low-density lipoprotein binding; protein binding; apolipoprotein binding; calcium ion binding; glycoprotein binding; calcium-dependent protein binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; nervous system development; positive regulation of protein kinase activity; ventral spinal cord development; negative regulation of transcription from RNA polymerase II promoter; lipid transport; signal transduction; memory

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 1