Rabbit anti-Human TUTase Polyclonal Antibody | anti-TUT1 antibody

TUTase Antibody

Cat. Num. AAA9602262

• Gene Names: TUT1; PAPD2; RBM21; TENT1; URLC6; TUTase; STARPAP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TUTase; Polyclonal Antibody; TUTase Antibody; alpha regulated poly(A) polymerase; nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I; nuclear speckle targeted PIPK1A regulated poly(A) polymerase; PAP associated domain containing 2; PAPD2; poly(A) polymerase associated domain containing 2; RBM21; RNA binding motif protein 21; RNA-binding motif protein 21; RNA-binding protein 21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; Star-PAP; STARPAP; STPAP_HUMAN; terminal uridylyl transferase 1; U6 snRNA specific; TUT1; U6 snRNA specific terminal uridylyltransferase 1; U6 snRNA-specific terminal uridylyltransferase 1; U6 TUTase; U6-TUTase; URLC6; anti-TUT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TUTase antibody detects endogenous levels of total TUTase
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 912

• Applicable Applications for anti-TUT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000
• Immunogen: A synthesized peptide derived from human TUTase
• Subcellular Location: Nucleus > Nucleolus. Nucleus Speckle.
• Tissue Specificity: Widely expressed.
• Predicted Cross Reactivity: Bovine, Horse, Rabbit, Dog
• Similarity: Bovine (91%), Horse (100%), Rabbit (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of TUTase using 3T3 whole cell lysates)

Related Product Information for anti-TUT1 antibody

Function: Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
Subunit Structure: Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction.
Post-translational Modifications: Phosphorylated by CK1 in the proline-rich (Pro-rich) region.
Similarity: Belongs to the DNA polymerase type-B-like family.

NCBI and Uniprot Product Information

• NCBI GI #: 226371750
• NCBI GeneID: 64852
• NCBI Accession #: NP_073741.2
• NCBI GenBank Nucleotide #: NM_022830.2
• UniProt Accession #: Q9H6E5; Q2NL65; Q7L583; Q9H6H7; A1A527; A8K995
• Molecular Weight: Observed: 95 kDa; Predicted: 94 kDa
• NCBI Official Full Name: speckle targeted PIP5K1A-regulated poly(A) polymerase
• NCBI Official Synonym Full Names: terminal uridylyl transferase 1, U6 snRNA-specific
• NCBI Official Symbol: TUT1
• NCBI Official Synonym Symbols: PAPD2; RBM21; TENT1; URLC6; TUTase; STARPAP
• NCBI Protein Information: speckle targeted PIP5K1A-regulated poly(A) polymerase
• UniProt Protein Name: Speckle targeted PIP5K1A-regulated poly(A) polymerase
• Protein Family: Speckle targeted PIP5K1A-regulated poly(A) polymerase
• UniProt Gene Name: TUT1
• UniProt Synonym Gene Names: RBM21; Star-PAP; RNA-binding protein 21; U6-TUTase

NCBI Description

This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009]

Uniprot Description

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.