Rabbit anti-Human TUTase Polyclonal Antibody | anti-TUTase antibody

TUTase polyclonal antibody

Cat. Num. AAA3000568

• Gene Names: TUT1; PAPD2; RBM21; TUTase; STARPAP
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TUTase; Polyclonal Antibody; TUTase polyclonal antibody; TUT1; RBM21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; Star-PAP; RNA-binding motif protein 21; RNA-binding protein 21; U6 snRNA-specific terminal uridylyltransferase 1; U6-TUTase; anti-TUTase antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: TUTase Polyclonal Antibody detects endogenous levels of TUTase protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-TUTase antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/20,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human TUT1. AA range: 291-340
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using TUT1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Related Product Information for anti-TUTase antibody

Background: terminal uridylyl transferase 1, U6 snRNA-specific(TUT1) Homo sapiens. This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009]

NCBI and Uniprot Product Information

• NCBI GI #: 226371750
• NCBI GeneID: 64852
• NCBI Accession #: NP_073741.2
• NCBI GenBank Nucleotide #: NM_022830.2
• UniProt Accession #: Q9H6E5; Q2NL65; Q7L583; Q9H6H7; A1A527; A8K995
• Molecular Weight: 93,847 Da
• NCBI Official Full Name: speckle targeted PIP5K1A-regulated poly(A) polymerase
• NCBI Official Synonym Full Names: terminal uridylyl transferase 1, U6 snRNA-specific
• NCBI Official Symbol: TUT1
• NCBI Official Synonym Symbols: PAPD2; RBM21; TUTase; STARPAP
• NCBI Protein Information: speckle targeted PIP5K1A-regulated poly(A) polymerase; speckle targeted PIP5K1A-regulated poly(A) polymerase; star-PAP; U6 TUTase; U6-TUTase; RNA-binding protein 21; RNA uridylyltransferase; RNA binding motif protein 21; RNA-binding motif protein 21; PAP-
• UniProt Protein Name: Speckle targeted PIP5K1A-regulated poly(A) polymerase
• UniProt Gene Name: TUT1
• UniProt Synonym Gene Names: RBM21; Star-PAP; RNA-binding protein 21; U6-TUTase
• UniProt Entry Name: STPAP_HUMAN

NCBI Description

This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009]

Uniprot Description

RBM21: Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3'-end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Does not play a role in replication-dependent histone mRNA degradation.

Protein type: EC 2.7.7.19; Transferase; Nucleolus; EC 2.7.7.52

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; intercellular bridge; nucleolus; nuclear speck; mRNA cleavage and polyadenylation specificity factor complex

Molecular Function: polynucleotide adenylyltransferase activity; protein binding; enzyme binding; mRNA 3'-UTR binding; RNA binding; RNA uridylyltransferase activity; metal ion binding; ATP binding

Biological Process: snRNA processing; mRNA polyadenylation; mRNA cleavage