Rabbit anti-Mouse Tumor Protein p53 (TP53) Polyclonal Antibody | anti-TP53 antibody

Polyclonal Antibody to Tumor Protein p53 (TP53)

Cat. Num. AAA2005965

• Gene Names: Trp53; bbl; bfy; bhy; p44; p53; Tp53
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Tumor Protein p53 (TP53); Polyclonal Antibody; Polyclonal Antibody to Tumor Protein p53 (TP53); anti-TP53 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TP53. It has been selected for its ability to recognize TP53 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-TCPVQLW VSATPPAGSR VRAMAIYKKS QHMTEVVRRC PHHERCSDGD GLAPPQHLIR VEGNLYPEYL EDRQTFRHSV VVPYEPPEAG SEYTTIHYKY MCNSSCMGGM NRRPILTIIT LEDSSGNLLG RDSFEVRVCA CPGRDRRTEE ENFRKKEVLC PELPPGSAKR ALPTCTSASP PQKKKPLDGE YFTLKIRGRK RFEMFRELNE ALELKDAHAT EESGDSRAHS SYLKTKKGQS TSRHKKTMVK KVGPDSD
• Sequence Length: 393

• Applicable Applications for anti-TP53 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant TP53 (Thr134~Asp387) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2049586
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human 293T Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant TP53, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Lung Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Brain Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 187960040
• NCBI GeneID: 22059
• NCBI Accession #: NP_001120705.1
• NCBI GenBank Nucleotide #: NM_001127233.1
• UniProt Accession #: P02340; Q9QUP3
• Molecular Weight: 43,155 Da
• NCBI Official Full Name: cellular tumor antigen p53 isoform b
• NCBI Official Synonym Full Names: transformation related protein 53
• NCBI Official Symbol: Trp53
• NCBI Official Synonym Symbols: bbl; bfy; bhy; p44; p53; Tp53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• Protein Family: Cellular tumor antigen
• UniProt Gene Name: Tp53
• UniProt Synonym Gene Names: P53; Trp53

NCBI Description

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Protein type: Activator; DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 11 B3|11 42.83 cM

Cellular Component: chromatin; cytoplasm; cytosol; endoplasmic reticulum; intracellular; mitochondrial matrix; mitochondrion; nuclear body; nuclear chromatin; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body; protein complex; replication fork; transcription factor complex; transcription factor TFIID complex

Molecular Function: ATP binding; chaperone binding; chromatin binding; copper ion binding; damaged DNA binding; DNA binding; DNA binding transcription factor activity; enzyme binding; histone acetyltransferase binding; histone deacetylase binding; histone deacetylase regulator activity; identical protein binding; metal ion binding; mRNA 3'-UTR binding; p53 binding; protease binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein kinase binding; protein N-terminus binding; protein phosphatase 2A binding; protein phosphatase binding; protein self-association; receptor tyrosine kinase binding; sequence-specific DNA binding; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding

Biological Process: apoptosis; autophagy; B cell lineage commitment; cell aging; cell cycle; cell cycle arrest; cellular protein localization; cellular response to DNA damage stimulus; cellular response to gamma radiation; cellular response to glucose starvation; cellular response to ionizing radiation; cellular response to reactive oxygen species; cellular response to stress; cellular response to UV; cellular response to UV-C; central nervous system development; cerebellum development; chromatin assembly; chromosome organization and biogenesis; circadian behavior; circadian rhythm; determination of adult lifespan; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; DNA strand renaturation; double-strand break repair; embryonic development ending in birth or egg hatching; embryonic organ development; entrainment of circadian clock by photoperiod; ER overload response; G1 DNA damage checkpoint; gastrulation; heart development; in utero embryonic development; intrinsic apoptotic signaling pathway by p53 class mediator; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; mitochondrial DNA repair; mitotic cell cycle arrest; mRNA transcription; multicellular organism development; multicellular organism growth; necroptotic process; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of DNA biosynthetic process; negative regulation of DNA replication; negative regulation of fibroblast proliferation; negative regulation of gene expression; negative regulation of glucose catabolic process to lactate via pyruvate; negative regulation of mitophagy; negative regulation of mitotic cell cycle; negative regulation of neuroblast proliferation; negative regulation of proteolysis; negative regulation of reactive oxygen species metabolic process; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; neuron apoptosis; nucleotide-excision repair; oxidative stress-induced premature senescence; positive regulation of apoptosis; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell aging; positive regulation of cell cycle; positive regulation of cell cycle arrest; positive regulation of execution phase of apoptosis; positive regulation of gene expression; positive regulation of histone deacetylation; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of leukocyte migration; positive regulation of neuron apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein oligomerization; positive regulation of reactive oxygen species metabolic process; positive regulation of release of cytochrome c from mitochondria; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress; positive regulation of transcription, DNA-templated; programmed cell death; protein complex assembly; protein homotetramerization; protein import into nucleus, translocation; protein localization; protein stabilization; protein tetramerization; regulation of apoptosis; regulation of cell cycle; regulation of cell proliferation; regulation of cellular senescence; regulation of fibroblast apoptotic process; regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter; regulation of intracellular pH; regulation of intrinsic apoptotic signaling pathway by p53 class mediator; regulation of mitochondrial membrane permeability involved in apoptotic process; regulation of neuron apoptosis; regulation of tissue remodeling; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; release of cytochrome c from mitochondria; replicative senescence; response to drug; response to gamma radiation; response to oxidative stress; response to salt stress; response to UV; response to X-ray; rhythmic process; rRNA transcription; signal transduction by p53 class mediator; somitogenesis; T cell differentiation in thymus; T cell lineage commitment; T cell proliferation during immune response; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; viral process