Western Blot (WB)
(Western blot detection of p53 in 3T3 cell lysates using p53 Rabbit pAb(1:1000 diluted). Predicted band size:44kDa. Observed band size:53kDa.)
Rabbit anti-Mouse p53 Polyclonal Antibody | anti-Trp53 antibody
p53 Rabbit pAb
Gene Names
Trp53; bbl; bfy; bhy; p44; p53; Tp53
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
p53; Polyclonal Antibody; p53 Rabbit pAb; Cellular tumor antigen p53; bbl; bfy; bhy; p44; Tp53; anti-Trp53 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-Trp53 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of mouse p53
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of p53 in 3T3 cell lysates using p53 Rabbit pAb(1:1000 diluted). Predicted band size:44kDa. Observed band size:53kDa.)
Western Blot (WB)
(Western blot detection of p53 in 3T3 cell lysates using p53 Rabbit pAb(1:1000 diluted). Predicted band size:44kDa. Observed band size:53kDa.)
Related Product Information for anti-Trp53 antibody
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44kDa
NCBI Official Full Name
cellular tumor antigen p53 isoform b
NCBI Official Synonym Full Names
transformation related protein 53
NCBI Official Symbol
Trp53
NCBI Official Synonym Symbols
bbl; bfy; bhy; p44; p53; Tp53
NCBI Protein Information
cellular tumor antigen p53; p53 cellular tumor antigen; tumor suppressor p53; tumor supressor p53
UniProt Protein Name
Cellular tumor antigen p53
Protein Family
UniProt Gene Name
Tp53
UniProt Synonym Gene Names
P53; Trp53
UniProt Entry Name
P53_MOUSE
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Product Notes
The Trp53 tp53 (Catalog #AAA4752147) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53 Rabbit pAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 ICC/IF: 1:20 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the Trp53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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