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Western Blot (WB) (Western Blot; Sample: Recombinant TNFRSF12A, Human.)

Rabbit anti-Human Tumor Necrosis Factor Receptor Superfamily Polyclonal Antibody | anti-TNFRSF12A antibody

Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)

Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Tumor Necrosis Factor Receptor Superfamily; Polyclonal Antibody; Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily; Member 12A (TNFRSF12A); anti-TNFRSF12A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-TNFRSF12A antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Cross Reactivity
Human
Traits
Liquid
Immunogen
Recombinant TNFRSF12A (Ser24~Ala126 (Accession # Q9NP84)) expressed in E.coli (MBS2029598)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant TNFRSF12A, Human.)

Western Blot (WB) (Western Blot; Sample: Recombinant TNFRSF12A, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Prostate cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human TNFRSF12A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Human Prostate cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human TNFRSF12A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 12A
NCBI Official Synonym Full Names
TNF receptor superfamily member 12A
NCBI Official Symbol
TNFRSF12A
NCBI Official Synonym Symbols
FN14; CD266; TWEAKR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 12A
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 12A
UniProt Gene Name
TNFRSF12A
UniProt Synonym Gene Names
FN14; FGF-inducible 14; TweakR

Uniprot Description

TweakR: Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. Associates with TRAF1 and TRAF2, and probably also with TRAF3. By FGF1 and phorbol ester. Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cell surface; integral component of membrane; plasma membrane; ruffle

Molecular Function: protein binding

Biological Process: angiogenesis; positive regulation of apoptosis; positive regulation of axon extension; regulation of angiogenesis; substrate-dependent cell migration, cell attachment to substrate; tumor necrosis factor-mediated signaling pathway

Research Articles on TNFRSF12A

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Product Notes

The TNFRSF12A tnfrsf12a (Catalog #AAA2028487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Receptor Superfamily can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TNFRSF12A tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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