Rabbit anti-Human BLVRB Polyclonal Antibody | anti-BLVRB antibody

BLVRB (Flavin Reductase (NADPH), FR, Biliverdin Reductase B, BVR-B, Biliverdin-IX beta-Reductase, Green Heme-binding Protein, GHBP, NADPH-dependent Diaphorase, NADPH-flavin Reductase, FLR) (Biotin)

Cat. Num. AAA6371312

• Gene Names: BLVRB; FLR; BVRB; SDR43U1; HEL-S-10
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BLVRB; Polyclonal Antibody; BLVRB (Flavin Reductase (NADPH); FR; Biliverdin Reductase B; BVR-B; Biliverdin-IX beta-Reductase; Green Heme-binding Protein; GHBP; NADPH-dependent Diaphorase; NADPH-flavin Reductase; FLR) (Biotin); anti-BLVRB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BLVRB.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-BLVRB antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human BLVRB, aa1-206 (NP_000704.1).
• Immunogen Sequence: MAVKKIAIFGATGQTGLTTLAQAVQAGYEVTVLVRDSSRLPSEGPRPAHVVVGDVLQAADVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVACTSAFLLWDPTKVPPRLQAVTDDHIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDLGHFMLRCLTTDEYDGHSTYPSHQYQ
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of BLVRB expression in transfected 293T cell line by BLVRB polyclonal antibody. Lane 1: BLVRB transfected lysate (22.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-BLVRB antibody

Clearance of heme in mammals is a two step process starting with conversion of heme to biliverdin by heme oxygenase, followed by reduction of biliverdin to bilirubin by bilivredin reductase. Biliverdin IX b reductase (BLVRB) converts the B isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore BLVRB is especially important for fetal heme metabolism and clearance. BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but is present in other tissues. The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyses the NADPH-dependent reduction for a variety of flavins, such as riboflavin, FAD or FMN and methemoglobin. BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin a isomer but could also use the B isomer as substrate, BLVRB is specific for the B isomer.

Product Categories/Family for anti-BLVRB antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4502419
• NCBI GeneID: 645
• NCBI Accession #: NP_000704.1
• NCBI GenBank Nucleotide #: NM_000713.1
• UniProt Accession #: P30043; P32078; P53005; Q32LZ2; A6NKD8; B2R5C6
• Molecular Weight: 22,119 Da
• NCBI Official Full Name: flavin reductase (NADPH)
• NCBI Official Synonym Full Names: biliverdin reductase B
• NCBI Official Symbol: BLVRB
• NCBI Official Synonym Symbols: FLR; BVRB; SDR43U1; HEL-S-10
• NCBI Protein Information: flavin reductase (NADPH); flavin reductase (NADPH); BVR-B; FR; GHBP; NADPH-dependent diaphorase; NADPH-flavin reductase; biliverdin reductase B (flavin reductase (NADPH)); biliverdin-IX beta-reductase; epididymis secretory protein Li 10; green heme-bindin
• UniProt Protein Name: Flavin reductase (NADPH)
• UniProt Gene Name: BLVRB
• UniProt Synonym Gene Names: FLR; FR; BVR-B; GHBP; FLR
• UniProt Entry Name: BLVRB_HUMAN

NCBI Description

The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24).[supplied by OMIM, Jul 2009]

Uniprot Description

BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC 1.5.1.30; EC 1.3.1.24

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: flavin reductase activity; biliverdin reductase activity

Biological Process: heme catabolic process; porphyrin metabolic process