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Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Ovary Tissue; Lane3: Mouse Testis Tissue.)
Rabbit anti-Mouse Tubulin Delta (TUBd) Polyclonal Antibody | anti-TUBd antibody
Polyclonal Antibody to Tubulin Delta (TUBd)
Gene Names
Tubd1; Tubd; 4930550G19Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Tubulin Delta (TUBd); Polyclonal Antibody; Polyclonal Antibody to Tubulin Delta (TUBd); anti-TUBd antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TUBd. It has been selected for its ability to recognize TUBd in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GSVFQ PTYSEDSSFH YRRNPLGDLM EHLVPHPEFK MLGVRNIPQM SAASLAYSAF TWAGLLKHLR QMLISSAKME EGINWQVRPP LTGLPPIGKA SAHKEHHFNT SLANLVVLRG REVHSADVEG FKDPALYTSW LEPVDAFSVW KTQRAFDKYE KSAALVSNSQ LLVKPLDMIV GKAWNMFSSK AFIHQYTKFG MEEEDFLDSF ALLEQVVASY GSLGP
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GSVFQ PTYSEDSSFH YRRNPLGDLM EHLVPHPEFK MLGVRNIPQM SAASLAYSAF TWAGLLKHLR QMLISSAKME EGINWQVRPP LTGLPPIGKA SAHKEHHFNT SLANLVVLRG REVHSADVEG FKDPALYTSW LEPVDAFSVW KTQRAFDKYE KSAALVSNSQ LLVKPLDMIV GKAWNMFSSK AFIHQYTKFG MEEEDFLDSF ALLEQVVASY GSLGP
Sequence Length
421
Applicable Applications for anti-TUBd antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TUBd (Gly236~Pro455) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075193
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Ovary Tissue; Lane3: Mouse Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Ovary Tissue; Lane3: Mouse Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TUBd, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TUBd, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,023 Da
NCBI Official Full Name
tubulin delta chain isoform 2
NCBI Official Synonym Full Names
tubulin, delta 1
NCBI Official Symbol
Tubd1
NCBI Official Synonym Symbols
Tubd; 4930550G19Rik
NCBI Protein Information
tubulin delta chain
UniProt Protein Name
Tubulin delta chain
UniProt Gene Name
Tubd1
Uniprot Description
TUBD1: In the elongating spermatid it is associated with the manchette, a specialized microtubule system present during reshaping of the sperm head. Belongs to the tubulin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 11|11 C
Cellular Component: cytoplasm; cytoskeleton; cytosol; microtubule; nucleoplasm; nucleus
Molecular Function: GTP binding; GTPase activity; nucleotide binding; structural constituent of cytoskeleton
Biological Process: cell differentiation; microtubule-based process; multicellular organism development; spermatogenesis
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Product Notes
The TUBd tubd1 (Catalog #AAA2007075) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tubulin Delta (TUBd) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin Delta (TUBd) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TUBd tubd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GSV FQ PTYSEDSSFH YRRNPLGDLM EHLVPHPEFK MLGVRNIPQM SAASLAYSAF TWAGLLKHLR QMLISSAKME EGINWQVRPP LTGLPPIGKA SAHKEHHFNT SLANLVVLRG REVHSADVEG FKDPALYTSW LEPVDAFSVW KTQRAFDKYE KSAALVSNSQ LLVKPLDMIV GKAWNMFSSK AFIHQYTKFG MEEEDFLDSF ALLEQVVASY GSLGP. It is sometimes possible for the material contained within the vial of "Tubulin Delta (TUBd), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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