Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Tubulin Epsilon (TUBe) Polyclonal Antibody | anti-TUBe antibody
Polyclonal Antibody to Tubulin Epsilon (TUBe)
Gene Names
Tube1; Tube; AI551343; 2310061K05Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Tubulin Epsilon (TUBe); Polyclonal Antibody; Polyclonal Antibody to Tubulin Epsilon (TUBe); anti-TUBe antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TUBe. It has been selected for its ability to recognize TUBe in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PKSLI TSNMGAVKKH HKKPFDAMNN IVANLLLSLT SSARFEGSLN MDLNEISMNL VPFPKLHYLV SSLTPLYTLA DVNIPPRRLD QMFSDAFSKD HQLIQADPRH SLYLACALIV RGNVQISDLR RNIERLKPAL QFVSWNQEGW KTSLCSVPPV GHSHSLLALA NNTCVKPTFM ELRERFTRLY KKKAHLHHYL QVDGMEESTF TEAVSSLSAL IQEYSDLDAT KSLPVPDVPR LSV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PKSLI TSNMGAVKKH HKKPFDAMNN IVANLLLSLT SSARFEGSLN MDLNEISMNL VPFPKLHYLV SSLTPLYTLA DVNIPPRRLD QMFSDAFSKD HQLIQADPRH SLYLACALIV RGNVQISDLR RNIERLKPAL QFVSWNQEGW KTSLCSVPPV GHSHSLLALA NNTCVKPTFM ELRERFTRLY KKKAHLHHYL QVDGMEESTF TEAVSSLSAL IQEYSDLDAT KSLPVPDVPR LSV
Sequence Length
447
Applicable Applications for anti-TUBe antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TUBe (Pro236~Val473) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075211
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded brain tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,652 Da
NCBI Official Full Name
tubulin epsilon chain
NCBI Official Synonym Full Names
epsilon-tubulin 1
NCBI Official Symbol
Tube1
NCBI Official Synonym Symbols
Tube; AI551343; 2310061K05Rik
NCBI Protein Information
tubulin epsilon chain
UniProt Protein Name
Tubulin epsilon chain
UniProt Gene Name
Tube1
Uniprot Description
TUBE1: a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5.[provided by RefSeq, Jan 2009]
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 10|10 B1
Cellular Component: cytoplasm; cytoskeleton; microtubule
Molecular Function: GTP binding; GTPase activity; nucleotide binding
Biological Process: microtubule-based process
Similar Products
Product Notes
The TUBe tube1 (Catalog #AAA2005458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Tubulin Epsilon (TUBe) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin Epsilon (TUBe) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TUBe tube1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PKS LI TSNMGAVKKH HKKPFDAMNN IVANLLLSLT SSARFEGSLN MDLNEISMNL VPFPKLHYLV SSLTPLYTLA DVNIPPRRLD QMFSDAFSKD HQLIQADPRH SLYLACALIV RGNVQISDLR RNIERLKPAL QFVSWNQEGW KTSLCSVPPV GHSHSLLALA NNTCVKPTFM ELRERFTRLY KKKAHLHHYL QVDGMEESTF TEAVSSLSAL IQEYSDLDAT KSLPVPDVPR LSV. It is sometimes possible for the material contained within the vial of "Tubulin Epsilon (TUBe), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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