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Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Rat Heart Tissue; Lane3: Human Hela Cells.)
Rabbit anti-Mouse Peptidylprolyl Isomerase F (PPIF) Polyclonal Antibody | anti-PPIF antibody
Polyclonal Antibody to Peptidylprolyl Isomerase F (PPIF)
Gene Names
Ppif; CypD; CyP-D; CyP-F; PPIase; AW457192
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Peptidylprolyl Isomerase F (PPIF); Polyclonal Antibody; Polyclonal Antibody to Peptidylprolyl Isomerase F (PPIF); anti-PPIF antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PPIF. It has been selected for its ability to recognize PPIF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPLVYLD VGADGQPLGR VVLELKADVV PKTAENFRAL CTGEKGFGYK GSTFHRVIPA FMCQAGDFTN HNGTGGRSIY GSRFPDENFT LKHVGPGVLS MANAGPNTNG SQFFICTIKT DWLDGKHVVF GHVKEGMDVV
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPLVYLD VGADGQPLGR VVLELKADVV PKTAENFRAL CTGEKGFGYK GSTFHRVIPA FMCQAGDFTN HNGTGGRSIY GSRFPDENFT LKHVGPGVLS MANAGPNTNG SQFFICTIKT DWLDGKHVVF GHVKEGMDVV
Sequence Length
207
Applicable Applications for anti-PPIF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PPIF (Asn44~Lys192) expressed in E.coli.
Cross Reactivity
Human, Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056420
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Rat Heart Tissue; Lane3: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Rat Heart Tissue; Lane3: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PPIF, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PPIF, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Brain Tissue)
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Hela cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Hela cells))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,737 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F (cyclophilin F)
NCBI Official Symbol
Ppif
NCBI Official Synonym Symbols
CypD; CyP-D; CyP-F; PPIase; AW457192
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
Protein Family
UniProt Gene Name
Ppif
UniProt Synonym Gene Names
PPIase F; CyP-D; CypD
Uniprot Description
PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.
Protein type: Cyclophilin; EC 5.2.1.8; Isomerase; Mitochondrial
Chromosomal Location of Human Ortholog: 14|14 A3
Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrial permeability transition pore complex; mitochondrion
Molecular Function: cyclosporin A binding; isomerase activity; peptide binding; peptidyl-prolyl cis-trans isomerase activity; protein binding
Biological Process: apoptosis; apoptotic mitochondrial changes; negative regulation of apoptosis; negative regulation of ATPase activity; programmed cell death; protein folding; protein peptidyl-prolyl isomerization; regulation of apoptosis; regulation of mitochondrial membrane permeability; response to oxidative stress
Research Articles on PPIF
Similar Products
Product Notes
The PPIF ppif (Catalog #AAA2017799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Peptidylprolyl Isomerase F (PPIF) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Peptidylprolyl Isomerase F (PPIF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPLVYLD VGADGQPLGR VVLELKADVV PKTAENFRAL CTGEKGFGYK GSTFHRVIPA FMCQAGDFTN HNGTGGRSIY GSRFPDENFT LKHVGPGVLS MANAGPNTNG SQFFICTIKT DWLDGKHVVF GHVKEGMDVV. It is sometimes possible for the material contained within the vial of "Peptidylprolyl Isomerase F (PPIF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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