Rabbit anti-Human, rat Tubulin b Polyclonal Antibody

Anti-Tubulin beta antibody

Cat. Num. AAA355105

• Reactivity: Human, rat
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: Tubulin b; Polyclonal Antibody; Anti-Tubulin beta antibody; Polyclonal Anti- Tubulin; anti-Tubulin b antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-Tubulin b antibody: Western Blot (WB)
• Application Notes: WB (1:2000)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of human Tubulin b.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Western Blot (WB)

Related Product Information for anti-Tubulin b antibody

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of alpha- and beta-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8.1 Mutant huntingtin, disrupted intracellular transport and insulin secretion by direct interference with microtubular beta-tubulin. Mutant huntingtin impaired glucose-stimulated insulin secretion in insulin-producing beta cells, without altering stored levels of insulin. Smith et al. (2009) proposed a novel pathogenetic process by which mutant huntingtin may disrupt hormone exocytosis from beta cells and possibly impair vesicular transport in any cell that expresses the pathogenic protein.2

References

1. Williams RC Jr, Shah C, Sackett D (November 1999). "Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels". Anal Biochem 275 (2): 265-7. 2. Smith, R., Bacos, K., Fedele, V., Soulet, D., Walz, H. A., Obermuller, S., Lindqvist, A., Bjorkqvist, M., Klein, P., Onnerfjord, P., Brundin, P., Mulder, H., Li, J.-Y. Mutant huntingtin interacts with beta-tubulin and disrupts vesicular transport and insulin secretion. Hum. Molec. Genet. 18: 3942-3954, 2009.