Rabbit TUBA3C/TUBA3D/TUBA3E Polyclonal Antibody | anti-TUBA3C antibody

TUBA3C/TUBA3D/TUBA3E Polyclonal Antibody

Cat. Num. AAA3007641

• Gene Names: TUBA3C; TUBA2; bA408E5.3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TUBA3C/TUBA3D/TUBA3E; Polyclonal Antibody; TUBA3C/TUBA3D/TUBA3E Polyclonal Antibody; Tubulin alpha-3C/D chain; Alpha-tubulin 2; Alpha-tubulin 3C/D; Tubulin alpha-2 chain; Detyrosinated tubulin alpha-3C/D chain; TUBA3C; TUBA2; TUBA3D; Tubulin alpha-3E chain; Alpha-tubulin 3E; Detyrosinated tubulin alpha-3E chain; TUBA3E; anti-TUBA3C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TUBA3C/TUBA3D/TUBA3E polyclonal antibody detects endogenous levels of TUBA3C/TUBA3D/TUBA3E protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 450

• Applicable Applications for anti-TUBA3C antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human TUBA3C.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TUBA3C/TUBA3D/TUBA3E polyclonal antibody at 1:500 dilutionLane1:The Brain tissue lysate of Rat (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:THP-1 whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:The Testis tissue lysate of Rat (40ug) Lane7:The Testis tissue lysate of Mouse (40ug))

Related Product Information for anti-TUBA3C antibody

Tubulin is a major cytoskeleton component that has five distinct forms, designated alpha, beta, gamma, delta and epsilon Tubulin. alpha and beta tubulins form heterodimers which multimerize to form a microtubule filament. There are 5 beta Tubulin isoforms (beta1, beta2, beta3, beta4A and beta4B) that are expressed in mammalian tissues. beta1 and beta4 are present throughout the cytosol, beta2 is present in the nuclei and nucleoplasm, and beta3 is a neuron-specific cytoskeletal protein. gamma Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both delta Tubulin and epsilon Tubulin are associated with the centrosome. delta Tubulin is a homolog of the Chlamydomonas delta Tubulin Uni3 and is found in association with the centrioles, whereas epsilon Tubulin localizes to the pericentriolar material. epsilon Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 17921993
• NCBI GeneID: 7278
• NCBI Accession #: NP_005992
• NCBI GenBank Nucleotide #: NP_005992.1
• UniProt Accession #: Q13748; Q6PEY2; P0DPH7
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: tubulin alpha-3C chain
• NCBI Official Synonym Full Names: tubulin alpha 3c
• NCBI Official Symbol: TUBA3C
• NCBI Official Synonym Symbols: TUBA2; bA408E5.3
• NCBI Protein Information: tubulin alpha-3C chain
• UniProt Protein Name: Tubulin alpha-3C/D chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA3C
• UniProt Synonym Gene Names: TUBA2
• UniProt Entry Name: TBA3C_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene is an alpha tubulin gene that encodes a protein 99% identical to the mouse testis-specific Tuba3 and Tuba7 gene products. This gene is located in the 13q11 region, which is associated with the genetic diseases Clouston hidrotic ectodermal dysplasia and Kabuki syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

TUBA3C/D: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Testis specific. Belongs to the tubulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q11

Cellular Component: microtubule; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; microtubule-based process