Rabbit TSC1 Polyclonal Antibody | anti-TSC1 antibody

TSC1 Antibody

Cat. Num. AAA9405982

• Gene Names: TSC1; LAM; TSC
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: TSC1; Polyclonal Antibody; TSC1 Antibody; Tuberous sclerosis complex 1; Hamartin; anti-TSC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1164

• Applicable Applications for anti-TSC1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.
• Target Name: TSC1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.)

Immunocytochemistry (ICC)

(Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 ug/mL)

Related Product Information for anti-TSC1 antibody

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Product Categories/Family for anti-TSC1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 2331281
• NCBI GeneID: 7248
• Molecular Weight: 124,015 Da
• NCBI Official Full Name: hamartin
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: TSC1
• NCBI Official Synonym Symbols: LAM; TSC
• NCBI Protein Information: hamartin
• UniProt Protein Name: Hamartin
• Protein Family: Tuberous sclerosis 1 protein
• UniProt Gene Name: TSC1
• UniProt Synonym Gene Names: KIAA0243; TSC
• UniProt Entry Name: TSC1_HUMAN

NCBI Description

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

Uniprot Description

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; growth cone; protein complex; membrane; intracellular membrane-bound organelle; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; GTPase activating protein binding; protein N-terminus binding; GTPase regulator activity

Biological Process: myelination; regulation of cell-matrix adhesion; protein heterooligomerization; regulation of cell cycle; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; cell projection organization and biogenesis; positive regulation of focal adhesion formation; synapse organization and biogenesis; cell cycle arrest; kidney development; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; glucose import; negative regulation of translation; regulation of stress fiber formation; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1