Goat Hamartin / TSC1 Polyclonal Antibody | anti-TSC1 antibody

Goat Polyclonal to Human Hamartin / TSC1

Cat. Num. AAA243410

• Gene Names: TSC1; LAM; TSC
• Reactivity: Gibbon, Dog, Gorilla, Horse, Human, Monkey, Orangutan, Rat; Predicted Reactivity: Mouse, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: Hamartin / TSC1; Polyclonal Antibody; Goat Polyclonal to Human Hamartin / TSC1; Anti-Hamartin / TSC1 Antibody (C-Terminus) IHC-plus; TSC1; LAM; Tuberous sclerosis 1 protein; Tuberous sclerosis 1; Hamartin; KIAA0243; TSC; Tumor suppressor; Human Hamartin; anti-TSC1 antibody

• Host: Goat
• Reactivity: Gibbon, Dog, Gorilla, Horse, Human, Monkey, Orangutan, Rat; Predicted Reactivity: Mouse, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TSC1. This antibody is expected to recognise isoform 1 (NP_000359.1) only.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1164

• Applicable Applications for anti-TSC1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QLHIMDYNETHHEH from the C-terminus of human TSC1 (NP_000359.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Panda, Dog, Horse, Platypus (100%); Mouse, Hamster, Turkey (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: Hamartin / TSC1 antibody was raised against synthetic peptide C-QLHIMDYNETHHEH from the C-terminus of human TSC1 (NP_000359.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Panda, Dog, Horse, Platypus (100%); Mouse, Hamster, Turkey (93%); Bat, Rabbit (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TSC1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TSC1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-TSC1 antibody

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors.

NCBI and Uniprot Product Information

• NCBI GI #: 4507693
• NCBI GeneID: 7248
• NCBI Accession #: NP_000359.1
• NCBI GenBank Nucleotide #: NM_000368.4
• UniProt Accession #: Q92574; Q5VVN5; B7Z897
• Molecular Weight: 124,015 Da
• NCBI Official Full Name: hamartin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: TSC1
• NCBI Official Synonym Symbols: LAM; TSC
• NCBI Protein Information: hamartin; tumor suppressor; tuberous sclerosis 1 protein
• UniProt Protein Name: Hamartin
• Protein Family: Hamartin
• UniProt Gene Name: TSC1
• UniProt Synonym Gene Names: KIAA0243; TSC
• UniProt Entry Name: TSC1_HUMAN

NCBI Description

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

Uniprot Description

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; protein complex; growth cone; membrane; intracellular membrane-bound organelle; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; GTPase activating protein binding; protein N-terminus binding; GTPase regulator activity

Biological Process: myelination; protein heterooligomerization; regulation of cell cycle; regulation of cell-matrix adhesion; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; cell projection organization and biogenesis; positive regulation of focal adhesion formation; synapse organization and biogenesis; cell cycle arrest; kidney development; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; regulation of stress fiber formation; negative regulation of translation; glucose import; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1