Rabbit anti-Human, Mouse TRMT1 Polyclonal Antibody | anti-TRMT1 antibody

TRMT1 Polyclonal Antibody

Cat. Num. AAA3008745

• Gene Names: TRMT1; TRM1; MRT68
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TRMT1; Polyclonal Antibody; TRMT1 Polyclonal Antibody; TRM1; tRNA (guanine(26)-N(2))-dimethyltransferase; anti-TRMT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRMT1 polyclonal antibody detects endogenous levels of TRMT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 630

• Applicable Applications for anti-TRMT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human TRMT1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using TRMT1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric using TRMT1 antibody at dilution of 1:100 (x40 lens).)

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using TRMT1 antibody.)

Related Product Information for anti-TRMT1 antibody

N(2), N(2)-dimethylguanosine tRNA methyltransferase, also known as TRMT1 or tRNA(guanine-26, N(2)-N(2)) methyltransferase, is a 659 amino acid enzyme that is responsible for tRNA modifications in eukaryotes. Using S-adenosyl-L-methionine as a methyl donor, TRMT1 dimethylates a single guanine residue at position 26 of tRNA. TRMT1, which was initially identified in yeast and C. elegans, has a 26% and 31% sequence identity to its yeast and C. elegans homologs, respectively. There are two isoforms of TRMT1 produced by alternative splicing events. The TRMT1 gene maps to chromosome 19p13.13 and mutations in this gene lead to abrogated enzyme activity and a decrease in protein levels.

NCBI and Uniprot Product Information

• NCBI GI #: 17390714
• NCBI GeneID: 55621
• UniProt Accession #: Q9NXH9
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: TRMT1 protein
• NCBI Official Synonym Full Names: tRNA methyltransferase 1
• NCBI Official Symbol: TRMT1
• NCBI Official Synonym Symbols: TRM1; MRT68
• NCBI Protein Information: tRNA (guanine(26)-N(2))-dimethyltransferase
• UniProt Protein Name: tRNA (guanine(26)-N(2))-dimethyltransferase
• Protein Family: TRMT1-like protein
• UniProt Gene Name: TRMT1
• UniProt Entry Name: TRM1_HUMAN

NCBI Description

This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome. [provided by RefSeq, May 2017]

Uniprot Description

Function: Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.

Catalytic activity: 2 S-adenosyl-L-methionine + guanine26 in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine26 in tRNA.

Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.Contains 1 C3H1-type zinc finger.Contains 1 Trm1 methyltransferase domain.